| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | RCL; C6orf108; dJ330M21.3 |
| WB Predicted band size | 19 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human DNPH1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于DNPH1抗体的参考文献摘要(文献标题及内容为虚构示例，仅供格式参考)：
1. **标题**: "DNPH1作为卵巢癌化疗敏感性的生物标志物研究"
**作者**: Zhang L, et al.
**摘要**: 本研究通过制备特异性DNPH1多克隆抗体，发现DNPH1在铂类耐药卵巢癌组织中表达显著下调。抗体免疫组化结果显示，DNPH1低表达与患者化疗反应差及生存期缩短相关，提示其可作为预测化疗敏感性的潜在标志物。
2. **标题**: "DNPH1抗体在DNA损伤修复机制中的功能验证"
**作者**: Tanaka K, et al.
**摘要**: 利用CRISPR/Cas9敲除细胞系及商业化DNPH1单克隆抗体，证实DNPH1通过水解8-oxo-dGMP防止DNA氧化损伤。Western blot和免疫荧光实验显示，DNPH1缺失导致γH2AX焦点聚集增加，表明其参与维持基因组稳定性。
3. **标题**: "新型DNPH1人源化抗体的开发及抗肿瘤应用"
**作者**: Wang Y, et al.
**摘要**: 研究团队开发了一种高亲和力人源化DNPH1抗体，通过体外实验证明该抗体可特异性靶向DNPH1蛋白并增强肿瘤细胞对PARP抑制剂的敏感性。动物模型显示抗体联合治疗显著抑制三阴性乳腺癌生长。
注：以上文献为模拟内容，实际研究中请通过PubMed或Web of Science查询真实文献(关键词：DNPH1 antibody, DPTH2. ovarian cancer, DNA repair)。
The DNPH1 (2'-deoxynucleoside 5'-phosphate N-hydrolase 1) antibody is a tool used to detect and study the DNPH1 protein, which plays a role in nucleotide metabolism. DNPH1. also known as C6orf130. is an enzyme that hydrolyzes 5'-deoxynucleoside phosphates to generate deoxyribose 5-phosphate and a nucleobase, contributing to the salvage pathway for nucleotide recycling. This function is critical in maintaining cellular nucleotide pools, particularly in environments with limited resources, such as hypoxic tumor tissues. DNPH1 has gained attention in cancer research due to its overexpression in certain malignancies, where it may support rapid cell proliferation by optimizing nucleotide availability.
Antibodies targeting DNPH1 are typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. These antibodies enable techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to analyze DNPH1 expression levels, localization, and regulation in cells or tissues. Researchers use these tools to explore DNPH1's involvement in cancer progression, DNA repair mechanisms, and cellular stress responses. Recent studies also suggest potential links between DNPH1 and chemotherapeutic resistance, making it a candidate biomarker or therapeutic target. Validation of DNPH1 antibodies includes specificity checks via knockout controls or siRNA silencing to ensure accurate detection in experimental models.
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