Chicken Cluster of Differentiation 4(CD4) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E13114C

Price： ￥3000

48T 96T

数量：

大包装询价

产品描述：
鸡CD4分子(CD4)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CD4含量。CD4是一种T细胞受体共受体，位于T细胞表面，与MHC II类分子结合，协助T细胞识别抗原。CD4在HIV感染中起关键作用，HIV病毒通过结合CD4进入T细胞，导致CD4+T细胞数量减少，免疫功能受损。研究CD4的功能和调控机制对于理解HIV感染和开发抗病毒药物具有重要意义。试剂盒检测范围为3.125 ng/mL-800 ng/mL，适用于科研领域中对鸡免疫系统的功能研究，包括感染性疾病模型中的T细胞应答分析、疫苗免疫效果评估以及免疫抑制性疾病机制探索等基础研究，为禽类免疫学实验提供可靠的定量检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：
CD4
Uniprot No.：
Q9W6V7
种属：
Gallus gallus (Chicken)
样本类型：
serum, plasma, tissue homogenates
检测范围：
3.125 ng/mL-800 ng/mL
灵敏度：
3.125 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken CD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-89
1:2	Average %	91
	Range %	87-96
1:4	Average %	92
	Range %	87-98
1:8	Average %	101
	Range %	97-105

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average
800	0.174	0.172	0.173
200	0.291	0.275	0.283
50	0.508	0.546	0.527
12.5	0.813	0.835	0.824
3.125	1.365	1.398	1.382
0	2.105	2.168	2.137