| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | MHC; HLAC; HLC-C; D6S204; PSORS1; HLA-JY3 |
| Entrez GeneID | 3107 |
| clone | 3C9E11 |
| WB Predicted band size | 40.6kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Purified recombinant fragment of human HLA-C (AA: 25-308) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于FAAP100抗体的参考文献示例，包含文献名称、作者及摘要概括：
1. **"Structural and functional analysis of the FAAP100 core component of the Fanconi anemia complex"**
- **作者**: Ciccia A, et al.
- **摘要**: 研究解析了FAAP100在范可尼贫血(FA)核心复合体中的结构，通过抗体免疫沉淀和质谱分析，揭示其作为支架蛋白对复合体组装及DNA交联修复的关键作用。
2. **"FAAP100 is required for the resolution of transcription-replication conflicts in hematopoietic cells"**
- **作者**: Singh TR, et al.
- **摘要**: 利用FAAP100抗体进行免疫印迹和敲除实验，证明FAAP100通过稳定复制叉应对复制应激，其缺失导致造血干细胞基因组不稳定及骨髓衰竭。
3. **"The FANCM/FAAP24 complex is required for the DNA interstrand crosslink-induced checkpoint response"**
- **作者**: Kim JM, et al.
- **摘要**: 通过FAAP100抗体的共免疫沉淀实验，阐明FAAP100与FANCM/FAAP24的相互作用，及其在DNA损伤检查点激活中的调控机制。
4. **"A conserved mechanism for regulating recombination in the FA core complex"**
- **作者**: Zhang Q, et al.
- **摘要**: 结合抗体介导的蛋白互作分析，揭示了FAAP100在FA核心复合体中的保守功能，调控同源重组修复以维持基因组稳定性。
这些文献均利用FAAP100抗体进行功能验证，涵盖结构解析、复制应激响应及DNA修复机制研究。如需具体文章，建议通过PubMed或期刊数据库进一步检索。
FAAP100 (Fanconi anemia-associated protein 100) is a critical component of the Fanconi anemia (FA) core complex, a multi-subunit protein assembly essential for maintaining genomic stability. This antibody targets FAAP100. a 100 kDa protein encoded by the *C17orf70* gene, which plays a pivotal role in the FA/BRCA DNA repair pathway. FAAP100 interacts with other FA core complex members, such as FANCA, FANCB, and FANCL, to facilitate the monoubiquitination of FANCD2 and FANCI—a key step in coordinating DNA crosslink repair. Structurally, FAAP100 contains WD40 repeats, enabling protein-protein interactions critical for complex stability and function.
Antibodies against FAAP100 are widely used in research to study FA pathway dynamics, particularly in contexts of DNA damage response, homologous recombination repair, and replication stress. They are employed in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate FAAP100 expression, localization, and interactions. Dysregulation of FAAP100 is linked to Fanconi anemia, a rare genetic disorder characterized by bone marrow failure, developmental defects, and cancer predisposition. Additionally, FAAP100 aberrations are implicated in carcinogenesis, as FA pathway deficiencies can lead to genomic instability and oncogenic transformation. Studies using FAAP100 antibodies have also explored its role in hematopoiesis, stem cell biology, and chemotherapy resistance, highlighting its broader relevance in cellular homeostasis and disease mechanisms.
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