Rabbit Polyclonal TTLL5 Antibody

The TTLL5 (Tubulin Tyrosine Ligase-Like 5) antibody is a research tool designed to study the TTLL5 protein, a member of the tubulin tyrosine ligase-like family. TTLL5 is an enzyme involved in post-translational modifications of tubulin, particularly the polyglutamylation of microtubules—a process critical for regulating microtubule stability, intracellular transport, and cellular structures like cilia and flagella. This protein contains a conserved tubulin tyrosine ligase (TTL) domain but functions as a polyglutamylase, adding glutamate chains to tubulin's C-terminal tails. Dysregulation of TTLL5 has been implicated in ciliopathies, neurodegenerative disorders, and cancer, as abnormal microtubule glutamylation disrupts cellular processes such as mitosis, neuronal development, and ciliary function. The TTLL5 antibody enables the detection and localization of TTLL5 in tissues or cultured cells via techniques like Western blotting, immunohistochemistry, and immunofluorescence. It is essential for investigating TTLL5's role in disease mechanisms, developmental biology, and microtubule dynamics. Researchers also utilize this antibody to explore potential therapeutic targets linked to microtubule-related pathologies. Validation of antibody specificity through knockout controls or siRNA knockdown is crucial to ensure accurate experimental outcomes.