| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HAP2; CBF-A; CBF-B; NF-YA |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human NFYA |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NFYA抗体的3-4篇文献摘要概述：
1. **"NF-YA regulates cell cycle progression by modulating miR-29b expression in glioblastoma"**
- **作者**: Dolfini et al.
- **摘要**: 研究揭示NFYA通过调控miR-29b影响胶质母细胞瘤的细胞周期进程。通过ChIP-seq和NFYA抗体验证，发现NFYA直接结合miR-29b启动子，抑制其表达，从而促进肿瘤细胞增殖。
2. **"The transcription factor NF-YA is essential for embryonic stem cell self-renewal"**
- **作者**: Oldfield et al.
- **摘要**: 文章利用NFYA抗体进行免疫沉淀和敲低实验，证明NFYA通过调控Oct4和Nanog等干性基因维持胚胎干细胞多能性，其缺失导致干细胞分化。
3. **"NF-YA mediates DNA damage repair through transcriptional activation of BRCA1"**
- **作者**: Benatti et al.
- **摘要**: 研究使用NFYA抗体验证其在乳腺癌细胞中的功能，发现NFYA结合BRCA1启动子并激活其转录，促进DNA损伤修复，与患者预后相关。
4. **"NF-YA dysfunction contributes to neurodegeneration in Alzheimer's disease models"**
- **作者**: Park et al.
- **摘要**: 通过免疫组化和NFYA抗体检测，发现阿尔茨海默病模型中NFYA表达降低，导致突触相关基因转录抑制，加速神经元退行性病变。
以上研究均通过NFYA抗体验证其在疾病或细胞机制中的关键作用，涵盖癌症、干细胞和神经疾病等领域。
The NFYA antibody is a research tool designed to detect Nuclear Transcription Factor Y Subunit A (NF-YA), a critical component of the NF-Y heterotrimeric transcription factor complex. NF-Y, composed of NF-YA, NF-YB, and NF-YC subunits, binds to the CCAAT box, a conserved DNA motif in gene promoters, to regulate transcription. NF-YA contains an evolutionarily conserved DNA-binding domain and plays a central role in stabilizing the complex's interaction with DNA. It is ubiquitously expressed and implicated in diverse cellular processes, including cell cycle regulation, apoptosis, and stress responses. Dysregulation of NF-YA has been linked to cancers, metabolic disorders, and developmental defects.
NFYA antibodies are widely used in molecular biology to study NF-YA expression, localization, and function. They enable techniques like Western blotting, immunohistochemistry, chromatin immunoprecipitation (ChIP), and immunofluorescence. These antibodies are typically raised in rabbits or mice, with monoclonal and polyclonal variants available. Specificity validation via knockout controls or siRNA knockdown is essential due to potential cross-reactivity with homologous proteins. Researchers employ NFYA antibodies to explore its role in gene regulatory networks, disease mechanisms, and therapeutic targeting. Recent studies highlight NF-YA's involvement in cancer progression, stem cell differentiation, and metabolic reprogramming, underscoring its value as a biomarker or intervention target in precision medicine.
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