首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子胆固醇酯转移蛋白(CETP)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CETP含量。CETP（胆固醇酯转运蛋白）是一种血浆糖蛋白，其功能是促进胆固醇酯从高密度脂蛋白（HDL）转移到低密度脂蛋白（LDL）和极低密度脂蛋白（VLDL），从而影响血脂水平。CETP抑制剂通过抑制CETP活性，增加血清HDL-C水平并降低LDL-C水平，被视为治疗血脂异常的潜在方法。试剂盒检测范围为1.56 ng/mL-100 ng/mL，科研人员可将其应用于高脂血症模型兔的血清标志物分析、脂蛋白代谢通路研究或针对CETP抑制剂的药效学评价，可为脂质代谢机制研究、动物模型评估及药物开发中CETP活性调控分析提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CETPCholesteryl ester transfer protein ELISA kit; Lipid transfer protein I ELISA kit; Fragment ELISA kit
缩写：CETP
Uniprot No.：P22687
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CETP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 99 | ||||
| Range % | 94-102 | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 94-100 | |||||
| 1:400 | Average % | 83 | ||||
| Range % | 80-86 | |||||
| 1:800 | Average % | 91 | ||||
| Range % | 87-95 | |||||
回收率：
| The recovery of rabbit CETP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 97-103 | ||||
| EDTA plasma (n=4) | 84 | 81-89 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 1000.0820.0860.084 500.1690.1780.174 250.2410.2580.250 12.50.3610.3780.370 6.250.5820.5410.562 3.120.8780.8980.888 1.561.2081.2311.220 02.0152.0682.042 | ||||||
货期：3-5 working days
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