| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HLD10; P5CR2 |
| WB Predicted band size | 34 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PYCR2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PYCR2抗体的3篇示例参考文献(均为假设性示例，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: *"PYCR2-mediated proline synthesis promotes tumor growth in colorectal cancer"*
**作者**: Zhang L, et al.
**摘要**: 本研究利用PYCR2特异性抗体，通过免疫组化和Western blot分析发现PYCR2在结直肠癌组织中高表达，并证实其通过促进脯氨酸合成增强癌细胞增殖和抗凋亡能力。抗体验证实验表明PYCR2表达水平与患者预后负相关。
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2. **文献名称**: *"Functional characterization of PYCR2 in neuronal development using CRISPR/Cas9 and antibody-based approaches"*
**作者**: Kim S, et al.
**摘要**: 作者通过CRISPR/Cas9敲低PYCR2并结合免疫荧光(使用兔源PYCR2单克隆抗体)，发现PYCR2缺失导致神经元线粒体形态异常和突触功能受损，提示其在神经发育中的关键作用。
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3. **文献名称**: *"PYCR2 as a metabolic regulator in Alzheimer’s disease: Insights from antibody-based proteomic analysis"*
**作者**: Wang Y, et al.
**摘要**: 该研究采用PYCR2多克隆抗体对阿尔茨海默病患者脑组织进行蛋白质组学分析，发现PYCR2表达下降与线粒体代谢紊乱和淀粉样蛋白沉积相关，提示其可能成为神经退行性疾病的潜在治疗靶点。
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如需真实文献，建议在PubMed、Google Scholar等平台检索关键词“PYCR2 antibody”或“PYCR2 function”，并筛选近年高影响力期刊文章。
PYCR2 (pyrroline-5-carboxylate reductase 2) is an enzyme encoded by the PYCR2 gene, part of the PYCR family involved in proline biosynthesis. It catalyzes the reduction of pyrroline-5-carboxylate (P5C) to proline, a critical process for cellular homeostasis, collagen synthesis, and redox balance. PYCR2 localizes to mitochondria and is highly expressed in metabolically active tissues, including the brain, linking it to mitochondrial function and energy metabolism. Dysregulation of PYCR2 has been implicated in neurological disorders, such as hypomyelinating leukodystrophy and Alzheimer’s disease, as well as cancer, where proline metabolism supports tumor growth and stress adaptation.
PYCR2 antibodies are immunological tools designed to detect and quantify PYCR2 protein levels in research applications like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies aid in studying PYCR2's role in cellular processes, disease mechanisms, and metabolic pathways. Many PYCR2 antibodies are raised in rabbits or mice using specific antigenic peptides, with validation steps (e.g., knockout controls) ensuring specificity. Recent studies using PYCR2 antibodies have highlighted its interaction with mitochondrial complexes and potential as a biomarker in cancer prognosis. However, variability in antibody performance across experimental conditions underscores the need for rigorous validation. Research on PYCR2 continues to explore its therapeutic potential in metabolic and neurodegenerative diseases.
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