首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠补体因子H(CFH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CFH含量。CFH即补体因子 H，是补体替代途径的重要调节蛋白。其在维持补体系统平衡、保护宿主细胞免受补体攻击等方面有重要作用。研究机制上，主要围绕其与补体成分相互作用，调节补体激活过程展开，异常时可能引发多种疾病。试剂盒检测范围为31.25 ng/mL-2000 ng/mL，该产品可广泛应用于小鼠模型研究中CFH蛋白的动态监测，例如自身免疫性疾病机制探索、感染模型补体通路分析、肾脏或眼部疾病相关研究等领域，为评估基因敲除/过表达模型、药物干预效果或病理进程中的补体调控机制提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cfh ELISA kit; Hf1Complement factor H ELISA kit; Protein beta-1-H ELISA kit
缩写：CFH
Uniprot No.：P06909
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 ng/mL-2000 ng/mL
灵敏度：7.81 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CFH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 92 | ||||
| Range % | 88-95 | |||||
| 1:2000 | Average % | 98 | ||||
| Range % | 93-101 | |||||
| 1:4000 | Average % | 87 | ||||
| Range % | 84-90 | |||||
| 1:8000 | Average % | 98 | ||||
| Range % | 92-102 | |||||
回收率：
| The recovery of mouse CFH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 88-97 | ||||
| EDTA plasma (n=4) | 98 | 93-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected20002.5522.6132.5832.46610002.0282.0072.0181.9015001.5071.5131.5101.3932500.8150.8220.8190.7021250.4970.4880.4930.37662.50.2950.2870.2910.17431.250.2080.2210.2150.09800.1160.1180.117 | ||||||
货期：3-5 working days
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