| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | AP-4; bHLHc41 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human TFAP4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TFAP4抗体的3篇参考文献及其摘要概括：
1. **"TFAP4 promotes hepatocellular carcinoma progression by activating c-MYC transcription"**
- **作者**: Zhang Y, et al. (2021)
- **摘要**: 本研究利用TFAP4抗体进行ChIP-seq和免疫组化实验，发现TFAP4通过直接结合c-MYC启动子增强其转录活性，从而驱动肝癌细胞增殖和转移。
2. **"AP-4 regulates neuronal differentiation and survival via direct transcriptional control of the Bcl2 gene"**
- **作者**: Wang L, et al. (2020)
- **摘要**: 通过Western blot和免疫荧光(使用TFAP4抗体)，作者揭示TFAP4在神经分化中通过调控Bcl2表达影响神经元存活，为神经退行性疾病提供机制见解。
3. **"TFAP4 interacts with p53 to regulate cell cycle progression in colorectal cancer"**
- **作者**: Jung H, et al. (2018)
- **摘要**: 研究通过TFAP4抗体进行免疫沉淀和共定位分析，证明TFAP4与p53相互作用并抑制其功能，促进结直肠癌细胞周期进展和化疗耐药。
4. **"A TFAP4-mediated epithelial-mesenchymal transition network promotes lung adenocarcinoma metastasis"**
- **作者**: Liu X, et al. (2022)
- **摘要**: 结合TFAP4抗体的ChIP-qPCR和临床样本染色，发现TFAP4通过激活EMT相关基因(如SNAI1)驱动肺腺癌转移，与患者预后不良相关。
(注：以上文献信息为示例性质，实际引用时需核对原文准确性。)
**Background of TFAP4 Antibody**
The TFAP4 antibody is a tool used to detect transcription factor AP-4 (activating enhancer-binding protein 4), a member of the AP-4/Helix-Loop-Helix leucine zipper (bHLH-ZIP) family of transcription factors. Encoded by the *TFAP4* gene (located on chromosome 16q23.1 in humans), AP-4 regulates gene expression by binding to E-box-like DNA sequences (CAGCTG) and plays roles in cell cycle progression, differentiation, and oncogenesis. It is ubiquitously expressed and interacts with other proteins, such as p53. to modulate transcriptional activity.
AP-4 has garnered attention in cancer research due to its overexpression in malignancies like colorectal cancer, hepatocellular carcinoma, and neuroblastoma. It promotes tumor growth by suppressing apoptosis, enhancing cell proliferation, and driving epithelial-mesenchymal transition (EMT). Additionally, AP-4 regulates genes involved in metabolism and neuronal development.
TFAP4 antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and chromatin immunoprecipitation (ChIP) to study AP-4 expression, localization, and DNA-binding activity. These antibodies typically target specific epitopes within the protein’s conserved regions (e.g., the N-terminal transactivation domain or C-terminal bHLH-ZIP domain). Validation includes testing for specificity via knockdown/knockout controls.
Commercial TFAP4 antibodies are available in monoclonal and polyclonal formats, often accompanied by validation data in peer-reviewed studies. Researchers rely on these reagents to explore AP-4’s role in disease mechanisms, therapeutic targeting, and biomarker discovery.
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