| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PKNOX1 |
| Uniprot No | P55347 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-436aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MMATQTLSID SYQDGQQMQV VTELKTEQDP NCSEPDAEGV SPPPVESQTP MDVDKQAIYR HPLFPLLALL FEKCEQSTQG SEGTTSASFD VDIENFVRKQ EKEGKPFFCE DPETDNLMVK AIQVLRIHLL ELEKVNELCK DFCSRYIACL KTKMNSETLL SGEPGSPYSP VQSQQIQSAI TGTISPQGIV VPASALQQGN VAMATVAGGT VYQPVTVVTP QGQVVTQTLS PGTIRIQNSQ LQLQLNQDLS ILHQDDGSSK NKRGVLPKHA TNVMRSWLFQ HIGHPYPTED EKKQIAAQTN LTLLQVNNWF INARRRILQP MLDSSCSETP KTKKKTAQNR PVQRFWPDSI ASGVAQPPPS ELTMSEGAVV TITTPVNMNV DSLQSLSSDG ATLAVQQVMM AGQSEDESVD STEEDAGALA PAHISGLVLE NSDSLQ |
| 预测分子量 | 48 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PKNOX1重组蛋白的参考文献(内容基于真实研究领域知识模拟，具体文献需通过学术数据库验证)：
1. **文献名称**: "Structural and Functional Analysis of PKNOX1 Recombinant Protein in Embryonic Development"
**作者**: Smith J, et al.
**摘要**: 本研究通过大肠杆菌系统表达并纯化了PKNOX1重组蛋白，解析了其与DNA结合的晶体结构，发现其通过同源结构域调控胚胎发育相关基因的转录活性。
2. **文献名称**: "PKNOX1 Recombinant Protein Modulates Hematopoietic Stem Cell Differentiation"
**作者**: Lee H, et al.
**摘要**: 利用昆虫细胞表达体系制备PKNOX1重组蛋白，发现其通过调控Wnt/β-catenin信号通路影响造血干细胞的自我更新和分化，揭示了其在血液系统疾病中的潜在作用。
3. **文献名称**: "Biochemical Characterization of PKNOX1 Interaction with Chromatin Remodeling Complexes"
**作者**: García-Ruiz C, et al.
**摘要**: 通过哺乳动物细胞表达PKNOX1重组蛋白，结合免疫共沉淀实验证明其与SWI/SNF染色质重塑复合物相互作用，阐明了其在表观遗传调控中的分子机制。
4. **文献名称**: "Development of a High-Yield PKNOX1 Recombinant Protein Production System for Drug Screening"
**作者**: Tanaka K, et al.
**摘要**: 优化PKNOX1在HEK293细胞中的重组表达条件，建立了高纯度制备工艺，并应用于高通量筛选靶向PKNOX1的小分子抑制剂，为癌症治疗提供新策略。
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**提示**：以上文献信息为模拟生成，建议通过PubMed、Web of Science或Google Scholar以关键词"PKNOX1 recombinant protein"检索最新研究，或关注领域内权威期刊(如*Nature Structural & Molecular Biology*、*Cell Reports*)。
PKNOX1 (PBX/knotted 1 homeobox 1), also known as PREP1. is a member of the TALE (three-amino acid loop extension) family of homeodomain-containing transcription factors. It plays a critical role in regulating gene expression during embryonic development, cell differentiation, and tissue homeostasis. Structurally, PKNOX1 contains a conserved homeodomain that facilitates DNA binding and a PBC (PBX-binding conserved) domain, enabling interaction with PBX (pre-B-cell leukemia homeobox) proteins. These PKNOX1-PBX heterodimers bind to specific DNA motifs, often collaborating with other transcription factors or co-regulators to modulate target genes involved in cell proliferation, apoptosis, and developmental patterning. Dysregulation of PKNOX1 has been implicated in developmental disorders and cancers, highlighting its importance in maintaining cellular balance.
Recombinant PKNOX1 protein is engineered through molecular cloning, typically expressed in bacterial (e.g., *E. coli*) or mammalian systems to ensure proper folding and post-translational modifications. The protein is purified via affinity tags (e.g., His-tag) and validated for functionality using assays like electrophoretic mobility shift assays (EMSAs) or luciferase reporter systems. In research, recombinant PKNOX1 serves as a tool to study its DNA-binding properties, protein-protein interactions, and regulatory mechanisms in vitro or in cell-based models. It is also used to explore therapeutic strategies, such as screening small molecules that modulate its activity in diseases linked to PKNOX1 dysfunction.
Overall, recombinant PKNOX1 bridges structural, functional, and translational studies, offering insights into its role in development and disease while enabling the development of targeted interventions.
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