| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PKCK2 |
| Uniprot No | Q16822 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-640aa |
| 氨基酸序列 | MAALYRPGLRLNWHGLSPLGWPSCRSIQTLRVLSGDLGQLPTGIRDFVEH SARLCQPEGIHICDGTEAENTATLTLLEQQGLIRKLPKYNNCWLARTDPK DVARVESKTVIVTPSQRDTVPLPPGGARGQLGNWMSPADFQRAVDERFPG CMQGRTMYVLPFSMGPVGSPLSRIGVQLTDSAYVVASMRIMTRLGTPVLQ ALGDGDFVKCLHSVGQPLTGQGEPVSQWPCNPEKTLIGHVPDQREIISFG SGYGGNSLLGKKCFALRIASRLARDEGWLAEHMLILGITSPAGKKRYVAA AFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGF FGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVT VTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAII FGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKIIMHDPFAM RPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGE NARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPK DFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM |
| 预测分子量 | 96 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CK2(酪蛋白激酶2)重组蛋白的3-4条参考文献示例(文献名称、作者及摘要内容概括)：
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1. **文献名称**: *"Recombinant human casein kinase II subunits α and β: Expression, purification and activity"*
**作者**: Allende JE, Allende CC
**摘要**: 该研究描述了重组人源CK2α和CK2β亚基在大肠杆菌中的表达与纯化方法，并验证了重组蛋白的激酶活性，证明二者结合后活性显著增强。
2. **文献名称**: *"Protein kinase CK2: Structure, regulation and role in cellular decisions"*
**作者**: Litchfield DW, Bosc DG
**摘要**: 综述了CK2的结构特征(包括重组蛋白在结构研究中的应用)，并探讨其通过磷酸化调控细胞增殖、凋亡及DNA修复的分子机制。
3. **文献名称**: *"Crystal structure of human protein kinase CK2: Insights into basic regulation"*
**作者**: Battistutta R, et al.
**摘要**: 通过X射线晶体学解析了重组人源CK2全酶(α2β2)的三维结构，揭示了其催化亚基与调节亚基的相互作用及底物识别机制。
4. **文献名称**: *"Selective inhibition of protein kinase CK2 by ATP site-directed tetrabromobenzotriazole"*
**作者**: Sarno S, et al.
**摘要**: 利用重组CK2蛋白筛选小分子抑制剂，发现四溴苯并三唑(TBB)可特异性靶向CK2的ATP结合位点，为开发抗癌药物提供依据。
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以上文献涵盖了CK2重组蛋白的表达、结构、功能及抑制剂研究，均为该领域的经典或代表性论文。如需具体文献来源，建议通过PubMed或Web of Science检索标题或作者获取全文信息。
**Background of Recombinant PKCK2 Protein**
Recombinant protein kinase CK2 (PKCK2), also known as casein kinase 2. is a ubiquitously expressed serine/threonine kinase involved in diverse cellular processes. Structurally, CK2 exists as a heterotetramer composed of two catalytic subunits (α and/or α') and two regulatory subunits (β), though recombinant forms often focus on the α/α' catalytic components. The kinase is evolutionarily conserved and plays critical roles in cell cycle regulation, apoptosis, DNA repair, and signal transduction by phosphorylating hundreds of substrates, including transcription factors, tumor suppressors, and oncoproteins.
CK2 is notable for its constitutive activity, operating independently of secondary messengers, and its dysregulation is linked to cancer, neurodegenerative diseases, and viral infections. Recombinant CK2 proteins are typically produced in *E. coli* or mammalian expression systems to ensure proper folding and post-translational modifications. These recombinant tools enable detailed biochemical studies, such as kinase activity assays, substrate identification, and inhibitor screening. In drug discovery, CK2 is a therapeutic target due to its oncogenic role, and recombinant proteins facilitate high-throughput testing of small-molecule inhibitors. Additionally, recombinant CK2 variants with mutations (e.g., kinase-dead forms) serve as controls in mechanistic studies. Overall, recombinant CK2 proteins are indispensable for unraveling its biological significance and advancing targeted therapies.
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