| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PIN4 |
| Uniprot No | Q9Y237-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-156aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMPMAGLLKGLVRQLEQFRVQQQASKMPPKG KSGSGKAGKGGAASGSDSADKKAQGPKGGGNAVKVRHILCEKHGKIMEAM EKLKSGMRFNEVAAQYSEDKARQGGDLGWMTRGSMVGPFQEAAFALPVSG MDKPVFTDPPVKTKFGYHIIMVEGRK |
| 预测分子量 | 19 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PIN4重组蛋白的3篇模拟参考文献示例(内容为假设性概括，供参考)：
1. **文献名称**：Structural Insights into PIN4 Recombinant Protein in Arabidopsis Auxin Transport
**作者**：Smith J., et al.
**摘要**：本研究解析了拟南芥PIN4重组蛋白的晶体结构，揭示其跨膜结构域对生长素极性运输的调控机制，为植物发育研究提供分子基础。
2. **文献名称**：Expression and Functional Analysis of Human PIN4 Recombinant Protein in Cell Cycle Regulation
**作者**：Chen L., et al.
**摘要**：通过大肠杆菌系统表达并纯化人源PIN4重组蛋白，证实其作为肽酰脯氨酰异构酶参与细胞周期检查点调控，可能与癌症病理相关。
3. **文献名称**：Optimization of PIN4 Recombinant Production in Yeast for Enzymatic Activity Assays
**作者**：Gomez R., et al.
**摘要**：开发了一种基于毕赤酵母的高效PIN4重组蛋白表达体系，验证其磷酸酶相互作用活性，为药物筛选提供可靠工具。
注：以上文献为模拟生成，实际引用需查询PubMed、Google Scholar等数据库获取真实研究。
**Background of PIN4 Recombinant Protein**
The PIN4 (Peptidyl-prolyl *cis-trans* isomerase NIMA-interacting 4) recombinant protein is a genetically engineered version of the native PIN4 protein, which plays a multifaceted role in cellular processes. Originally identified in *Saccharomyces cerevisiae* as a regulator of mitotic checkpoint control, PIN4 homologs are conserved across eukaryotes. The human ortholog, PIN4 (also called Rotamase or PPIase), belongs to the parvulin family of prolyl isomerases, enzymes that catalyze the *cis-trans* isomerization of peptide bonds preceding proline residues—a critical step in protein folding and conformational regulation.
PIN4 interacts with the mitotic kinase NIMA (Never In Mitosis A) in fungi, linking it to cell cycle progression and stress response pathways. Studies highlight its involvement in modulating phosphorylation-dependent signaling networks, including the ATM/ATR DNA damage response and tau protein homeostasis in neurons. Its recombinant form is typically produced in bacterial or eukaryotic expression systems, enabling structural and functional studies.
Research on recombinant PIN4 has unveiled its dual functional domains: an N-terminal TPR (tetratricopeptide repeat) motif for protein-protein interactions and a C-terminal catalytic domain for isomerase activity. Dysregulation of PIN4 is implicated in neurodegenerative diseases (e.g., Alzheimer’s via tau aggregation) and cancer, where altered cell cycle control promotes genomic instability.
The development of PIN4 recombinant protein has facilitated drug discovery efforts, particularly in designing inhibitors targeting its isomerase activity. Additionally, it serves as a tool to dissect mechanisms underlying protein misfolding diseases and mitotic regulation. Despite progress, its context-dependent roles in cellular stress adaptation and cross-talk with other isomerases remain active areas of investigation.
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