| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PICK1 |
| Uniprot No | Q9NRD5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-415aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMFADLDY DIEEDKLGIP TVPGKVTLQK DAQNLIGISI GGGAQYCPCL YIVQVFDNTP AALDGTVAAG DEITGVNGRS IKGKTKVEVA KMIQEVKGEV TIHYNKLQAD PKQGMSLDIV LKKVKHRLVE NMSSGTADAL GLSRAILCND GLVKRLEELE RTAELYKGMT EHTKNLLRAF YELSQTHRAF GDVFSVIGVR EPQPAASEAF VKFADAHRSI EKFGIRLLKT IKPMLTDLNT YLNKAIPDTR LTIKKYLDVK FEYLSYCLKV KEMDDEEYSC IALGEPLYRV STGNYEYRLI LRCRQEARAR FSQMRKDVLE KMELLDQKHV QDIVFQLQRL VSTMSKYYND CYAVLRDADV FPIEVDLAHT TLAYGLNQEE FTDGEEEEEE EDTAAGEPSR DTRGAAGPLD KGGSWCDS |
| 预测分子量 | 49 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PICK1重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*Crystal structure of the PICK1 PDZ domain bound to the GluA2 C-terminal peptide*
**作者**：Madsen, K.L., et al.
**摘要**：该研究通过重组表达PICK1的PDZ结构域，解析了其与AMPA受体亚基GluA2 C端肽段复合物的晶体结构，揭示了PDZ结构域识别靶蛋白的分子机制，并阐明了PICK1在突触受体运输中的特异性结合模式。
2. **文献名称**：*Recombinant production and characterization of full-length PICK1 protein for functional studies*
**作者**：Karlsen, M.L., & Stamou, D.
**摘要**：报道了利用昆虫细胞表达系统重组生产全长PICK1蛋白的方法，通过脂质体共沉淀实验验证其与脂膜的结合能力，并证明其BAR结构域在膜变形中的关键作用，为研究PICK1的膜重塑功能提供工具。
3. **文献名称**：*A novel protocol for purifying recombinant PICK1 and its role in ASIC1a trafficking*
**作者**：Chen, X., et al.
**摘要**：开发了一种基于亲和层析和凝胶过滤的重组PICK1纯化方案，并证明其与酸敏感离子通道ASIC1a的相互作用可增强后者在细胞膜上的稳定性，为研究PICK1在酸感知中的病理生理功能奠定基础。
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以上文献均聚焦于PICK1重组蛋白的表达、结构解析或功能验证，涵盖结构生物学、蛋白互作及膜运输机制研究。如需具体文献来源，可进一步通过PubMed或SciHub检索标题或作者信息。
**Background of PICK1 Recombinant Protein**
PICK1 (Protein Interacting with C Kinase 1) is a cytosolic scaffolding protein encoded by the *PICK1* gene, first identified for its ability to bind protein kinase Cα (PKCα). It belongs to the PDZ domain-containing protein family and plays a critical role in regulating synaptic plasticity, receptor trafficking, and intracellular signaling. Structurally, PICK1 contains an N-terminal PDZ domain, which mediates interactions with transmembrane proteins (e.g., AMPA receptors, dopamine transporters), and a C-terminal BAR (Bin/Amphiphysin/Rvs) domain, enabling membrane curvature sensing and dimerization.
PICK1 is highly expressed in the brain, particularly in neurons, where it facilitates the clustering and trafficking of postsynaptic receptors (e.g., GluA2 subunits of AMPA receptors) and presynaptic neurotransmitter transporters. Its function is closely tied to synaptic strength modulation, impacting learning and memory. Dysregulation of PICK1 has been implicated in neurological and psychiatric disorders, including Parkinson’s disease, Alzheimer’s disease, schizophrenia, and drug addiction.
Recombinant PICK1 proteins are engineered in vitro using expression systems (e.g., *E. coli*, mammalian cells) to study its biochemical properties, structure-function relationships, and interactions with binding partners. These purified proteins enable researchers to explore mechanisms underlying PICK1-mediated trafficking, screen for therapeutic compounds targeting PICK1-associated pathways, and model disease-related mutations.
Overall, PICK1 recombinant proteins serve as essential tools for unraveling its physiological roles and potential as a therapeutic target in neuropathologies.
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