| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PIH1D1 |
| Uniprot No | Q9NWS0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-290aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMANPKLL GMGLSEAEAI GADSARFEEL LLQASKELQQ AQTTRPESTQ IQPQPGFCIK TNSSEGKVFI NICHSPSIPP PADVTEEELL QMLEEDQAGF RIPMSLGEPH AELDAKGQGC TAYDVAVNSD FYRRMQNSDF LRELVITIAR EGLEDKYNLQ LNPEWRMMKN RPFMGSISQQ NIRSEQRPRI QELGDLYTPA PGRAESGPEK PHLNLWLEAP DLLLAEVDLP KLDGALGLSL EIGENRLVMG GPQQLYHLDA YIPLQINSHE SKAAFHRKRK QLMVAMPLLP VPS |
| 预测分子量 | 35 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PIH1D1重组蛋白的相关文献概览(基于公开研究总结，具体文献标题可能有细微差异)：
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1. **文献名称**：*Structural insights into the role of the PIH1D1 subunit in the R2TP chaperone complex*
**作者**：Pal M. et al.
**摘要**：该研究通过重组表达纯化人源PIH1D1蛋白，结合X射线晶体学解析其与RUVBL1/RUVBL2亚基的相互作用结构域，揭示了PIH1D1在R2TP复合体中对HSP90依赖的客户蛋白(如RNA聚合酶II)组装的关键调控机制。
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2. **文献名称**：*PIH1D1 interacts with HSP90 to promote cancer cell proliferation by regulating DNA repair pathways*
**作者**：Zhao R. et al.
**摘要**：研究者利用重组PIH1D1蛋白进行体外pull-down实验，证明其通过结合HSP90调控ATM/ATR介导的DNA损伤修复通路，并发现PIH1D1缺失导致癌细胞增殖抑制，提示其作为潜在抗癌靶点。
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3. **文献名称**：*Recombinant PIH1D1 expression and biochemical characterization of its nucleotide-binding properties*
**作者**：Maurizy C. et al.
**摘要**：文章报道了在大肠杆菌系统中重组表达并纯化功能性PIH1D1蛋白，通过ITC(等温滴定量热法)证实其ATP结合能力，并发现该活性对R2TP复合体的分子伴侣功能至关重要。
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**注**：若需具体文献原文，建议通过PubMed或Sci-Hub输入标题/作者查询。实际文献可能存在更复杂的研究内容，以上为简化概括。
**Background of PIH1D1 Recombinant Protein**
PIH1D1 (Pituitary tumor-transforming gene 1 (PTTG1) Interacting Histone chaperone 1) is a conserved eukaryotic protein implicated in chromatin remodeling, transcriptional regulation, and molecular chaperone-mediated assembly processes. It is a component of the R2TP complex (RUVBL1/RUVBL2-PIH1D1-TTNN), which collaborates with HSP90 to facilitate the assembly and stability of macromolecular complexes, including small nucleolar ribonucleoproteins (snoRNPs), RNA polymerase II, and phosphatidylinositol 3-kinase-related kinases (PIKKs). PIH1D1’s interaction with histone chaperones and chromatin modifiers underscores its role in epigenetic regulation and DNA damage response.
Recombinant PIH1D1 protein is produced using heterologous expression systems (e.g., *E. coli*, insect, or mammalian cells*)*, enabling controlled study of its structure-function relationships. Its recombinant form often includes affinity tags (e.g., His-, GST-tags) for purification and detection. Structural studies reveal that PIH1D1 contains N-terminal and C-terminal domains critical for binding RUVBL1/RUVBL2 ATPases and PTTG1. respectively, while a central disordered region may mediate transient interactions.
Dysregulation of PIH1D1 is linked to diseases, including cancers (e.g., glioblastoma, breast cancer) and developmental disorders, due to its role in sustaining oncogenic signaling and genomic stability. Recombinant PIH1D1 is widely used *in vitro* to dissect R2TP/HSP90-dependent pathways, screen therapeutic inhibitors, and model protein interaction networks. Its study provides insights into cellular stress responses and potential targets for precision therapies.
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