| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PINK1 |
| Uniprot No | Q9BXM7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 156-507aa |
| 氨基酸序列 | MYLIGQSIGK GCSAAVYEAT MPTLPQNLEV TKSTGLLPGR GPGTSAPGEG QERAPGAPAF PLAIKMMWNI SAGSSSEAIL NTMSQELVPA SRVALAGEYG AVTYRKSKRG PKQLAPHPNI IRVLRAFTSS VPLLPGALVD YPDVLPSRLH PEGLGHGRTL FLVMKNYPCT LRQYLCVNTP SPRLAAMMLL QLLEGVDHLV QQGIAHRDLK SDNILVELDP DGCPWLVIAD FGCCLADESI GLQLPFSSWY VDRGGNGCLM APEVSTARPG PRAVIDYSKA DAWAVGAIAY EIFGLVNPFY GQGKAHLESR SYQEAQLPAL PESVPPDVRQ LVRALLQREA SKRPSARVAA NVL |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PINK1重组蛋白的模拟参考文献示例(注：内容为虚构合成，仅作格式参考)：
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1. **标题**: *Recombinant Human PINK1 Purification and Characterization of its Autophosphorylation Activity*
**作者**: Kondapalli C, et al.
**摘要**: 本研究报道了人源PINK1重组蛋白在大肠杆菌中的高效表达及纯化方法，证实其具有依赖Mn²⁺的丝氨酸/苏氨酸激酶活性，并揭示了其N端结构域对线粒体膜电位下降后的自磷酸化调控机制。
2. **标题**: *Structural Insights into PINK1 Kinase Domain and its Interaction with Ubiquitin*
**作者**: Rasool S, et al.
**摘要**: 通过X射线晶体学解析了PINK1激酶结构域的重组蛋白三维结构，揭示了其与泛素结合的关键残基，为帕金森病相关突变导致功能异常的分子机制提供了结构基础。
3. **标题**: *Functional Reconstitution of PINK1-Parkin Signaling Using Recombinant Proteins in Vitro*
**作者**: Narendra DP, Youle RJ.
**摘要**: 利用重组PINK1和Parkin蛋白在体外重建了线粒体自噬信号通路，证明PINK1通过磷酸化泛素及Parkin激活下游泛素化级联反应，为药物筛选提供了体外模型。
4. **标题**: *Development of a FRET-Based Assay for High-Throughput Screening of PINK1 Activators*
**作者**: Chen Y, et al.
**摘要**: 基于重组PINK1蛋白构建了荧光共振能量转移(FRET)检测系统，用于快速筛选增强其激酶活性的小分子化合物，为神经退行性疾病治疗策略开发奠定基础。
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提示：实际文献需通过PubMed/Google Scholar检索关键词(如"recombinant PINK1 protein" + "kinase activity"/"purification")，并结合高影响力期刊(如*Nature*, *Cell*, *JBC*)筛选。
PINK1 (PTEN-induced kinase 1) is a serine/threonine-protein kinase encoded by the *PINK1* gene, located on human chromosome 1p36.12. It plays a critical role in mitochondrial quality control, particularly in mitophagy—the selective degradation of dysfunctional mitochondria. Under normal conditions, PINK1 is imported into healthy mitochondria and rapidly cleaved by proteases. However, upon mitochondrial depolarization or stress, PINK1 accumulates on the outer mitochondrial membrane, where it phosphorylates ubiquitin and the E3 ubiquitin ligase Parkin. This phosphorylation cascade recruits Parkin to damaged mitochondria, promoting their ubiquitination and subsequent autophagic clearance.
Mutations in the *PINK1* gene are linked to autosomal recessive early-onset Parkinson’s disease (PD), highlighting its importance in neuronal survival. Recombinant PINK1 protein, produced via expression systems like *E. coli* or mammalian cells, is widely used to study its structure, enzymatic activity, and interactions with substrates such as Parkin and ubiquitin. These studies aim to unravel molecular mechanisms underlying mitophagy dysregulation in neurodegenerative diseases.
Recombinant PINK1 typically includes tags (e.g., His-tag) for purification and tracking. Its applications span *in vitro* kinase assays, screening for kinase inhibitors or activators, and modeling PD pathogenesis. Recent research also explores PINK1's roles beyond mitophagy, including immune responses and cancer, broadening its therapeutic relevance. Despite progress, challenges remain in understanding its full substrate profile and regulatory pathways, driving continued demand for high-quality recombinant PINK1 tools.
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