| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | GMPR1; GMPR 1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human GMPR |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3篇与GMPR抗体相关的参考文献概览：
1. **"Characterization of a novel monoclonal antibody against human GMP reductase (GMPR) for cancer metabolism studies"**
- **作者**: Zhang Y, et al.
- **摘要**: 该研究开发了一种针对人源GMP还原酶(GMPR)的单克隆抗体，验证了其在Western blot和免疫荧光中的特异性。结果显示，GMPR在结直肠癌细胞中表达显著下调，可能与核苷酸代谢异常相关。
2. **"Immunohistochemical detection of GMPR in renal tissue: implications for diabetic nephropathy"**
- **作者**: Lee S, et al.
- **摘要**: 利用抗GMPR抗体对糖尿病肾病模型肾组织进行免疫组化分析，发现GMPR表达水平与氧化应激标志物呈负相关，提示其在肾脏代谢保护中的作用。
3. **"Development of a polyclonal antibody targeting plant GMP reductase and its application in stress response studies"**
- **作者**: Gupta R, et al.
- **摘要**: 研究报道了一种植物GMPR多克隆抗体的制备，通过ELISA和免疫印迹验证其效价，并应用于盐胁迫下拟南芥中GMPR的蛋白表达动态分析。
4. **"GMPR antibody-based proteomic profiling reveals metabolic adaptations in hepatocellular carcinoma"**
- **作者**: Wang X, et al.
- **摘要**: 通过抗GMPR抗体结合质谱技术，揭示了肝癌组织中GMPR的蛋白互作网络，发现其与嘌呤代谢通路的关键酶存在共表达，为肝癌治疗提供新靶点。
注：以上文献为示例性内容，实际引用需以具体数据库(如PubMed、Web of Science)检索结果为准。
**Background of GMPR Antibodies**
GMPR (Guanosine Monophosphate Reductase) is an enzyme critical in purine metabolism, catalyzing the NADPH-dependent conversion of GMP to IMP, a key step in nucleotide homeostasis. Dysregulation of GMPR has been implicated in metabolic disorders, immune dysfunction, and cancer, where altered nucleotide pools affect cell proliferation and survival.
GMPR antibodies are essential tools for studying this enzyme's expression, localization, and function. They enable detection via techniques like Western blotting, immunohistochemistry, and ELISA, aiding research into GMPR's role in diseases. For instance, reduced GMPR levels are linked to oxidative stress resistance in certain cancers, while polymorphisms correlate with autoimmune conditions like lupus.
These antibodies are typically developed against conserved epitopes in human GMPR (isoforms 1 and 2) and cross-react with homologs in model organisms. Recent studies also explore GMPR as a therapeutic target, with antibodies potentially guiding drug development. Challenges include ensuring specificity due to structural similarities with other oxidoreductases. Overall, GMPR antibodies bridge molecular insights and clinical applications in metabolic and oncological research.
×
