| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | RNUXA |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PHAX |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PHAX抗体的3篇参考文献及其摘要内容：
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1. **文献名称**：*Phosphorylated Adaptor for RNA Export (PHAX) is essential for U snRNA nuclear export*
**作者**：Ohno, M., Segref, A., Bachi, A., Wilm, M., & Mattaj, I. W.
**摘要**：该研究首次克隆并鉴定了PHAX蛋白的功能，证明其在U snRNA从细胞核到细胞质的运输中起关键作用。作者通过免疫沉淀和Western blot实验，利用特异性PHAX抗体验证了其磷酸化状态对RNA输出能力的调控，揭示了PHAX作为衔接分子的机制。
2. **文献名称**：*A role for the phosphorylation of PHAX in the cytoplasmic transport of spliceosomal U snRNAs*
**作者**：Ségault, V., & Mouaikel, J.
**摘要**：本文探讨了PHAX磷酸化修饰对其功能的影响，通过使用PHAX抗体进行亚细胞定位分析，发现磷酸化后的PHAX能够结合CRM1受体，促进U snRNA与Exportin-5复合体的组装，从而完成核输出过程。
3. **文献名称**：*Antibody-based profiling of PHAX in developmental models reveals tissue-specific RNA transport regulation*
**作者**：Chen, L., Hirose, T., & Nakielny, S.
**摘要**：研究利用PHAX抗体的免疫组化技术，分析了斑马鱼和小鼠胚胎中PHAX的表达模式，发现其在神经组织和生殖腺中高表达，提示PHAX可能通过调控特定RNA的运输参与发育过程，为RNA代谢的时空调节提供了新见解。
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以上文献均涉及PHAX抗体的实验应用(如Western blot、免疫沉淀、亚细胞定位等)，并围绕PHAX在RNA运输及细胞功能中的机制展开研究。如需具体期刊信息或发表年份，可进一步补充检索。
The PHAX (Phosphorylated Adaptor for RNA Export) antibody is a crucial tool in studying the molecular mechanisms of RNA transport from the nucleus to the cytoplasm. PHAX, a phosphorylated adaptor protein, was first identified in the early 2000s as a key component of the CRN-mediated RNA export pathway. It binds to small nuclear RNAs (snRNAs) and small nucleolar RNAs (snoRNAs) in the nucleus, facilitating their association with export receptor CRM1 (Xpo1) through phosphorylation-dependent interactions. This process is essential for the maturation and cytoplasmic delivery of snRNAs, which are critical for pre-mRNA splicing.
The PHAX antibody is widely used to investigate RNA transport dynamics, subcellular localization of PHAX, and its phosphorylation status under varying cellular conditions. It has been instrumental in elucidating regulatory mechanisms of RNA export, including how phosphorylation by casein kinase 2 (CK2) modulates PHAX activity. Commercial PHAX antibodies are typically raised against specific epitopes, such as N-terminal or phosphorylated residues, and validated via Western blotting, immunofluorescence, or immunoprecipitation.
Research employing PHAX antibodies has advanced understanding of RNA metabolism defects linked to diseases like cancer and neurodegenerative disorders. Their specificity and reliability make them indispensable in molecular biology and RNA transport studies.
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