| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | L19; QSK; SEMDK; SIK-3 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human SIK3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于SIK3抗体的3篇假设性参考文献示例(基于领域内典型研究方向，非真实文献)：
---
1. **文献名称**：*SIK3 regulates hepatic lipid homeostasis via phosphorylation of HDAC5*
**作者**：Horike N, et al.
**摘要**：本研究揭示了SIK3通过磷酸化组蛋白去乙酰化酶HDAC5调控肝脏脂代谢的机制。作者开发了一种特异性SIK3抗体，用于免疫沉淀和Western blot分析，证实了SIK3在肥胖小鼠模型中表达上调，并与其代谢功能直接相关。
---
2. **文献名称**：*Development and validation of a monoclonal antibody targeting human SIK3 for immunohistochemical applications*
**作者**：Clark KR, et al.
**摘要**：该文献报道了一种新型抗人SIK3单克隆抗体的制备与验证。通过ELISA、免疫荧光和免疫组化实验，作者证明该抗体具有高特异性和灵敏度，可用于检测多种组织样本中SIK3的定位及表达水平。
---
3. **文献名称**：*SIK3 inhibition sensitizes ovarian cancer cells to PARP inhibitors via DNA repair pathway modulation*
**作者**：Zhang Y, et al.
**摘要**：研究利用商业化SIK3抗体(如Abcam #ab12345)分析卵巢癌细胞中SIK3的表达与DNA修复的关系。结果表明，SIK3缺失会增强PARP抑制剂的疗效，提示其作为癌症治疗靶点的潜力。
---
**注意**：以上文献为示例，实际研究中请通过PubMed或Google Scholar搜索真实文献(关键词：SIK3 antibody, Salt-Inducible Kinase 3)。如需具体文章，可提供进一步研究方向(如疾病模型、实验技术等)。
The SIK3 (Salt-Inducible Kinase 3) antibody is a tool used to study the SIK3 protein, a member of the AMP-activated protein kinase (AMPK)-related kinase family. SIK3. encoded by the *SIK3* gene, plays a critical role in regulating cellular energy balance, lipid metabolism, and circadian rhythms. It is activated by phosphorylation in response to low energy states or hormonal signals, such as insulin and glucagon. SIK3 modulates downstream pathways by phosphorylating transcription co-regulators like CRTCs (CREB-regulated transcription coactivators) and class IIa histone deacetylases (HDACs), influencing gene expression linked to metabolic adaptation and stress responses.
Research highlights SIK3's involvement in diseases such as cancer, obesity, and bone disorders. For example, SIK3 overexpression is observed in certain ovarian and breast cancers, suggesting its role in tumor progression. In metabolic contexts, SIK3 regulates adipose tissue expansion and insulin sensitivity, making it a potential therapeutic target for obesity-related conditions.
SIK3 antibodies are essential for detecting protein expression, localization, and activity in tissues or cell lines via techniques like Western blotting, immunohistochemistry, and immunofluorescence. They enable studies on SIK3's mechanistic contributions to disease pathways and its interactions with signaling molecules. Recent drug discovery efforts also utilize these antibodies to validate SIK3 inhibitors in preclinical models. Overall, SIK3 antibodies are vital for advancing understanding of metabolic regulation and developing targeted therapies.
×
