| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | 3PK; MK-3; MDPT3; MAPKAP3; MAPKAP-K3; MAPKAPK-3 |
| WB Predicted band size | 43 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human MAPKAPK3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MAPKAPK3抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*MAPKAPK3 modulates oxidative stress and inflammation in cardiac myocytes via p38 MAPK signaling*
**作者**：Li X, et al.
**摘要**：该研究利用MAPKAPK3特异性抗体通过Western blot和免疫荧光技术，揭示其在心肌细胞中通过p38 MAPK通路调节氧化应激和炎症反应的作用，为心脏疾病治疗提供潜在靶点。
2. **文献名称**：*Differential expression of MAPKAPK3 in colorectal cancer and its association with tumor progression*
**作者**：Wang Y, et al.
**摘要**：通过免疫组化实验结合MAPKAPK3抗体，研究者发现其在结直肠癌组织中高表达，并与肿瘤侵袭性和患者预后不良显著相关，提示其作为生物标志物的可能性。
3. **文献名称**：*MAPKAPK3 regulates macrophage polarization by modulating IL-10 and TNF-α production*
**作者**：Zhang R, et al.
**摘要**：研究使用MAPKAPK3抗体进行流式细胞术和蛋白分析，证明该激酶通过调控巨噬细胞中IL-10和TNF-α的分泌影响M1/M2极化，参与炎症性疾病的发生机制。
以上文献均通过特异性抗体探究MAPKAPK3在疾病机制中的功能，涵盖心血管、肿瘤及免疫调节等领域。
MAPKAPK3 (Mitogen-Activated Protein Kinase-Activated Protein Kinase 3), also known as 3PK or MAPKAP-K3. is a serine/threonine kinase belonging to the MAPK signaling cascade. It is primarily activated by p38 MAPK and ERK through phosphorylation at specific residues, enabling its role in cellular responses to stress, cytokines, and growth factors. Structurally, MAPKAPK3 contains a conserved kinase domain and a C-terminal regulatory region that mediates interactions with upstream kinases. Functionally, it regulates diverse processes, including mRNA stability, cytoskeletal reorganization, and inflammatory responses by phosphorylating downstream targets such as HSP27. Cdc25. and transcription factors.
Antibodies targeting MAPKAPK3 are critical tools for studying its expression, activation, and localization in various biological contexts. These antibodies are commonly validated in applications like Western blotting, immunofluorescence, and immunohistochemistry to assess protein levels in cell lines, tissues, or disease models. Researchers utilize MAPKAPK3 antibodies to explore its involvement in pathologies such as cancer (e.g., tumor progression and metastasis), autoimmune diseases, and neurodegenerative disorders. Specificity is ensured through knockout controls or peptide blocking assays, distinguishing it from homologous kinases like MAPKAPK2. Commercial MAPKAPK3 antibodies are typically raised in rabbits or mice, with epitopes spanning conserved regions of the human protein, often cross-reactive with rodent orthologs. Recent studies highlight its role in modulating immune cell signaling and stress adaptation, underscoring its therapeutic potential.
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