首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠腱蛋白样蛋白1(CHRDL1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CHRDL1含量。小鼠腱蛋白样蛋白1（CHRDL1）是一种重要的分泌性发育介导蛋白，在多种组织中表达，具有调节细胞命运和促进神经元分化的功能。其研究机制涉及与BMP4结合作为拮抗剂，阻断受体相互作用，并可能参与多种生理和病理过程，如神经发育和癌症预后。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，本试剂盒适用于体外研究CHRDL1在神经生物学、骨骼发育或肿瘤模型中的表达水平变化。为探索CHRDL1的分子功能及调控机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Chrdl1 ELISA kit; Ng1 ELISA kit; Nrln1Chordin-like protein 1 ELISA kit; Neuralin-1 ELISA kit; Neurogenesin-1 ELISA kit; Ventroptin ELISA kit
缩写：CHRDL1
Uniprot No.：Q920C1
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CHRDL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-95 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 97-106 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 92-103 | |||||
| 1:8 | Average % | 86 | ||||
| Range % | 82-89 | |||||
回收率：
| The recovery of mouse CHRDL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-102 | ||||
| EDTA plasma (n=4) | 89 | 85-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.3772.3562.3672.2175001.5991.6871.6431.4932500.9330.9970.9650.8151250.5410.5680.5550.40562.50.3550.3260.3410.19131.20.2220.2360.2290.0797.80.1540.1670.1610.01100.1440.1560.150 | ||||||
货期：3-5 working days
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