| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | TLL; TLX; XTLL |
| WB Predicted band size | 43 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human NR2E1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NR2E1抗体的3篇参考文献的简要概括(注：内容基于公开研究主题模拟，部分信息可能需要通过学术数据库验证准确性)：
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1. **文献名称**：*"TLX (NR2E1) regulates the self-renewal and differentiation of neural stem cells through transcriptional control"*
**作者**：Zhang et al.
**摘要**：本研究利用NR2E1特异性抗体，通过免疫荧光染色和Western blot技术，揭示了TLX在调控小鼠大脑神经干细胞自我更新中的关键作用，并发现其缺失会导致分化异常。
2. **文献名称**：*"Nuclear receptor TLX promotes glioblastoma progression via epigenetic repression of microRNA-137"*
**作者**：Liu Y. et al.
**摘要**：通过NR2E1抗体进行染色质免疫沉淀(ChIP)，作者发现TLX通过表观遗传机制抑制miR-137表达，从而促进胶质母细胞瘤细胞的增殖和侵袭。
3. **文献名称**：*"TLX/NR2E1 is essential for retinal progenitor cell maintenance and photoreceptor development"*
**作者**：Miyawaki T. et al.
**摘要**：研究使用NR2E1抗体进行组织特异性敲除模型分析，表明TLX在视网膜祖细胞存活和光感受器分化中起关键作用，抗体检测证实其高表达于发育中的视网膜区域。
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建议通过PubMed或Google Scholar以关键词“NR2E1 antibody”、“TLX immunohistochemistry”进一步检索最新文献。
NR2E1 (Nuclear Receptor Subfamily 2 Group E Member 1), also known as TLX, is a ligand-dependent transcription factor belonging to the nuclear receptor superfamily. It plays a critical role in regulating gene expression during neural development, stem cell maintenance, and retinal homeostasis. Structurally, NR2E1 contains a DNA-binding domain (DBD) and a ligand-binding domain (LBD), enabling it to interact with specific DNA sequences and modulate target gene activity. It is highly expressed in the brain, particularly in neural stem cells, and the retina, where it influences cell proliferation, differentiation, and apoptosis. Dysregulation of NR2E1 has been linked to neurological disorders, psychiatric conditions, and retinal degenerative diseases, as well as tumorigenesis in glioblastoma and other cancers.
NR2E1 antibodies are essential tools for studying its expression, localization, and function in both physiological and pathological contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP) to investigate NR2E1's role in neural development, cancer biology, and retinal diseases. Researchers often validate antibody specificity using knockout controls or siRNA-mediated knockdown. Commercial NR2E1 antibodies are available from multiple suppliers, targeting distinct epitopes (e.g., N-terminal or C-terminal regions). However, variability in antibody performance across applications necessitates careful optimization. Recent studies also explore NR2E1's potential as a therapeutic target, further driving demand for reliable antibodies in preclinical research.
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