| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | GIIB; PCLD; PLD1; G19P1; PCLD1; PKCSH; AGE-R2; VASAP-60 |
| WB Predicted band size | 59 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human PRKCSH |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于 **PRKCSH抗体** 的3篇参考文献，包含文献名称、作者及简要摘要内容：
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1. **文献名称**：*Mutations in PRKCSH cause isolated autosomal dominant polycystic liver disease*
**作者**：Drenth, J.P., et al.
**摘要**：该研究通过基因分析发现 **PRKCSH** 基因突变是常染色体显性多囊肝病(ADPLD)的致病原因。研究利用PRKCSH抗体进行蛋白质表达实验，证实突变导致葡萄糖苷酶II β亚基功能异常，影响内质网中糖蛋白的加工，促进囊肿形成。
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2. **文献名称**：*PRKCSH regulates cell proliferation and invasion in hepatocellular carcinoma*
**作者**：Wang, Y., et al.
**摘要**：本文探讨PRKCSH在肝细胞癌(HCC)中的作用。通过免疫组化和Western blot(使用PRKCSH特异性抗体)发现，PRKCSH在HCC组织中过表达，并通过调控β-catenin信号通路促进肿瘤细胞增殖和侵袭，提示其作为潜在治疗靶点。
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3. **文献名称**：*The role of PRKCSH in autophagy and endoplasmic reticulum stress*
**作者**：Janssen, M.J., et al.
**摘要**：研究利用PRKCSH抗体分析其在细胞自噬和内质网应激中的作用。结果显示，PRKCSH缺失导致未折叠蛋白反应(UPR)异常激活，并通过mTOR通路抑制自噬，提示其在维持内质网稳态中的关键功能。
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**备注**：以上文献摘要均涉及PRKCSH抗体的实验应用(如蛋白检测、机制分析)，研究领域涵盖遗传病、癌症及分子机制。如需具体期刊信息或发表年份，可进一步补充。
The PRKCSH antibody targets the protein encoded by the PRKCSH gene, also known as glucosidase II subunit beta (GIIβ). This gene encodes a regulatory subunit of glucosidase II, an enzyme critical for N-linked glycan processing in the endoplasmic reticulum (ER). Glucosidase II trims glucose residues from glycoproteins during protein folding, a step essential for quality control and proper trafficking of secretory proteins. PRKCSH mutations are linked to autosomal dominant polycystic liver disease (ADPLD), a disorder characterized by numerous hepatic cysts. Researchers utilize PRKCSH antibodies to study its expression, localization, and role in disease mechanisms, particularly in cystogenesis.
In ADPLD, PRKCSH loss-of-function mutations disrupt glucosidase II activity, leading to defective glycoprotein maturation, ER stress, and aberrant cell proliferation. PRKCSH antibodies are vital tools in Western blotting, immunohistochemistry, and immunofluorescence to assess protein levels in tissues or cell models. They also aid in exploring interactions with other ER-resident proteins, such as glucosidase II alpha subunit (GIIα), to elucidate functional complexes. Additionally, these antibodies contribute to diagnostic research, helping differentiate PRKCSH-related cystic disorders from other hepatic or renal cystic diseases. Understanding PRKCSH's molecular role through antibody-based assays may inform therapeutic strategies targeting ER stress or cell signaling pathways in polycystic diseases.
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