首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人肌酸激酶同工酶MB(CK-MB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织匀浆样本中的CK-MB含量。CK-MB即肌酸激酶同工酶，主要存在于心肌中。它是诊断急性心肌梗死等心脏疾病的重要生物标志物。研究机制是当心肌受损时，CK-MB会释放进入血液，通过检测血中其含量变化，可辅助判断心肌损伤程度、范围及病情发展。试剂盒检测范围为0.9 ng/mL-60 ng/mL，本试剂盒适用于科研领域中心肌损伤相关疾病模型的机制探索，如心肌梗死、心肌炎或药物心脏毒性评估等研究场景，可通过检测不同样本类型（如血液样本动态监测病程进展，尿液样本探索新型标志物潜力，或组织匀浆分析离体心肌损伤）为心血管病理研究提供定量数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：CK-MB
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, tissue homogenates
检测范围：0.9 ng/mL-60 ng/mL
灵敏度：0.22 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 87 | |||||
| Range % | 84-90 | ||||||
| 1:2 | Average % | 104 | |||||
| Range % | 101-107 | ||||||
| 1:4 | Average % | 98 | |||||
| Range % | 94-102 | ||||||
| 1:8 | Average % | 101 | |||||
| Range % | 98-104 | ||||||
回收率：
| The recovery of human CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 85 | 81-89 | |||||
| EDTA plasma (n=4) | 109 | 104-113 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected602.0162.1062.0611.907301.6461.6041.6251.471151.2151.2531.2341.0807.50.8590.8480.8540.7003.750.5860.5520.5690.4151.80.3700.3670.3690.2150.90.2840.2780.2810.12700.1550.1530.154 | |||||||
货期：3-5 working days
×
