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产品描述：猪M型肌酸激酶(CKM)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CKM含量。猪M型肌酸激酶（CKM）是一种肌肉特异性酶，主要存在于骨骼肌细胞中，参与能量代谢。其研究机制涉及基因表达调控，特别是其启动子区域，通过特异性启动下游基因表达，影响肌肉生长和品质。研究旨在通过基因工程技术提高猪肉品质和生长速度。试剂盒检测范围为0.156 mU/mL-10 mU/mL，在科研领域，本产品适用于探究猪肌肉生理病理机制、运动代谢调控、肌肉相关疾病模型建立等研究，也可用于评估营养干预或药物处理对肌肉能量代谢的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CKMCreatine kinase M-type ELISA Kit; EC 2.7.3.2 ELISA Kit; Creatine kinase M chain ELISA Kit; Creatine phosphokinase M-type ELISA Kit; CPK-M ELISA Kit; M-CK ELISA Kit
缩写：CKM
Uniprot No.：Q5XLD3
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 mU/mL-10 mU/mL
灵敏度：0.039 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig CKM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:5 | Average % | 103 | |||||
| Range % | 92-107 | ||||||
| 1:10 | Average % | 98 | |||||
| Range % | 91-102 | ||||||
| 1:20 | Average % | 93 | |||||
| Range % | 84-97 | ||||||
| 1:40 | Average % | 99 | |||||
| Range % | 90-103 | ||||||
回收率：
| The recovery of pig CKM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 100 | 88-103 | |||||
| EDTA plasma (n=4) | 92 | 84-96 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| mU/mlOD1OD2AverageCorrected102.0591.9932.0261.89151.5571.6881.6231.4882.51.0811.1281.1050.9701.250.6220.6370.6300.4950.6250.3230.3310.3270.1920.3120.2150.2220.2190.0840.1560.1790.1730.1760.04100.1380.1320.135 | |||||||
货期：3-5 working days
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