| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/150-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | P28; hp28; dJ423B22.5 |
| WB Predicted band size | 30 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human DNALI1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DNALI1抗体的3篇参考文献示例(注：内容为虚构，仅用于演示格式)：
1. **"DNALI1 localization in mammalian sperm flagella and its role in motility"**
- **作者**: Smith A, et al.
- **摘要**: 本研究通过免疫荧光和Western blot技术，利用DNALI1抗体揭示了该蛋白在哺乳动物精子鞭毛中的特异性定位，并发现其表达缺失与精子运动障碍相关。
2. **"Characterization of DNALI1 mutations in primary ciliary dyskinesia patients"**
- **作者**: Chen L, et al.
- **摘要**: 文章通过DNALI1抗体分析患者呼吸道纤毛组织，发现部分原发性纤毛运动障碍(PCD)患者存在DNALI1基因突变，导致蛋白功能异常及纤毛结构缺陷。
3. **"DNALI1 as a component of the cytoplasmic dynein-2 complex in ciliary transport"**
- **作者**: Yamamoto K, et al.
- **摘要**: 利用DNALI1抗体进行免疫共沉淀实验，证实DNALI1是细胞质动力蛋白-2复合体的关键亚基，并参与纤毛内运输过程，影响 Hedgehog信号通路。
4. **"Antibody-based profiling of DNALI1 expression in human cancers"**
- **作者**: Gupta R, et al.
- **摘要**: 研究采用DNALI1抗体对多种癌症组织进行免疫组化分析，发现其表达水平在卵巢癌和肺癌中显著下调，提示其潜在肿瘤抑制作用。
(注：以上文献为示例，实际文献需通过PubMed/Google Scholar等平台检索。)
The DNALI1 antibody targets dynein light intermediate chain 1 (DNALI1), a component of the cytoplasmic dynein complex, a motor protein critical for intracellular cargo transport along microtubules. DNALI1. encoded by the *DNALI1* gene, functions as part of the dynein-dynactin machinery, facilitating retrograde vesicle trafficking, organelle positioning, and mitotic spindle organization. It plays a role in neuronal transport, ciliary function, and cell division. Dysregulation of DNALI1 has been linked to neurodevelopmental disorders and ciliopathies, such as primary ciliary dyskinesia (PCD), due to its importance in ciliary structure and motility.
Antibodies against DNALI1 are widely used in research to study dynein complex assembly, intracellular transport mechanisms, and disease pathology. They enable detection of DNALI1 expression via Western blotting, immunofluorescence, and immunohistochemistry, aiding in the characterization of cellular localization and protein interactions. Commercial DNALI1 antibodies are typically raised in rabbits or mice, with validation in specific applications (e.g., human or murine tissues). Researchers also utilize these antibodies to explore DNALI1's role in cancer progression, neurological diseases, and ciliary dysfunction. However, variability in antibody specificity requires careful validation to avoid cross-reactivity with related dynein subunits. Studies employing DNALI1 antibodies contribute to understanding cellular dynamics and developing therapeutic strategies for dynein-associated disorders.
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