| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | DDX8; Dhr2; HRH1; PRP22; PRPF22 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human DHX8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DHX8抗体的3篇参考文献示例(注：以下内容为模拟生成，实际文献可能存在差异，请以实际检索结果为准)：
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1. **文献名称**：*DHX8 is required for the release of spliced mRNA from the spliceosome*
**作者**：Koodathingal P, et al.
**摘要**：本研究利用DHX8特异性抗体通过免疫沉淀和Western blot分析，揭示了DHX8在剪接体拆卸中的关键作用。实验表明，DHX8通过其解旋酶活性促进成熟mRNA从剪接体复合物中释放，并参与剪接因子的回收。
2. **文献名称**：*DHX8 interacts with the Bact complex to regulate pre-mRNA splicing fidelity*
**作者**：Ahmad S, et al.
**摘要**：通过免疫共沉淀(使用DHX8抗体)和质谱分析，作者发现DHX8与剪接体Bact复合物直接互作，调控前体mRNA剪接的准确性。敲低DHX8导致异常剪接事件增多，提示其在维持剪接质量控制中的功能。
3. **文献名称**：*Dysregulation of DHX8 in cancer correlates with genomic instability*
**作者**：Sridhara SC, et al.
**摘要**：该研究通过免疫组化(IHC)和DHX8抗体检测发现，DHX8在多种癌症组织中表达异常。功能实验表明，DHX8缺失导致DNA损伤修复缺陷和染色体不稳定性，提示其作为潜在肿瘤标志物的可能性。
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如需获取真实文献，建议通过PubMed或Google Scholar检索关键词“DHX8 antibody”或“DHX8 helicase function”，并筛选涉及该抗体应用的实验研究。
The DHX8 antibody is a research tool designed to target the DEAH-box helicase 8 (DHX8) protein, a member of the DEAH-box RNA helicase family involved in pre-mRNA splicing and RNA metabolism. DHX8 plays a critical role in the later stages of spliceosome assembly, facilitating the release of mature mRNA from the spliceosome and ensuring proper RNA processing. Its function is essential for maintaining genomic stability and regulating gene expression.
Antibodies against DHX8 are widely used in molecular biology to study its expression, localization, and interaction partners in cellular pathways. They enable techniques like Western blotting, immunofluorescence, and immunoprecipitation, helping researchers investigate DHX8's role in diseases linked to splicing defects, such as cancer, neurodegenerative disorders, or viral infections. Some studies also explore DHX8's involvement in cellular stress responses or its potential as a therapeutic target.
These antibodies are typically raised in rabbits or mice using recombinant DHX8 protein fragments or synthetic peptides. Validation often includes testing in knockout cell lines to confirm specificity. Commercial DHX8 antibodies vary in clonality (monoclonal/polyclonal) and conjugates (e.g., HRP, fluorescent tags). Researchers must optimize experimental conditions due to potential cross-reactivity with related helicases. Recent applications include CRISPR-based studies and high-throughput screening for splicing-related drug discovery.
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