| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/150-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | EIF3S4; EIF3-P42; eIF3-p44; eIF3-delta |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human EIF3G |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于EIF3G抗体的虚构参考文献示例(仅供格式参考)：
1. **"EIF3G regulates cap-dependent translation and interacts with mTOR signaling in colorectal cancer"**
- Author: Zhang L, et al.
- 摘要：研究利用EIF3G抗体通过免疫沉淀和Western blot分析，揭示EIF3G在结直肠癌细胞中通过mTOR通路调控蛋白质翻译的机制，提示其作为潜在治疗靶点。
2. **"Development of a monoclonal antibody against EIF3G for detecting viral translation machinery hijacking"**
- Author: Kim S, et al.
- 摘要：报道一种新型EIF3G单克隆抗体的开发，验证其在检测RNA病毒(如HCV)劫持宿主翻译复合体中的特异性，为病毒感染机制研究提供工具。
3. **"EIF3G expression correlates with poor prognosis in glioblastoma multiforme"**
- Author: Patel R, et al.
- 摘要：通过免疫组化(使用EIF3G抗体)分析胶质母细胞瘤组织，发现EIF3G高表达与患者生存期缩短相关，提示其作为预后标志物的潜力。
4. **"Functional characterization of EIF3G in embryonic development using CRISPR/Cas9 and antibody-based assays"**
- Author: Müller J, et al.
- 摘要：结合CRISPR基因编辑和EIF3G抗体功能实验，证明EIF3G缺失导致小鼠胚胎发育中蛋白质合成障碍及细胞周期停滞。
注：以上文献为示例，实际引用需查询真实数据库(如PubMed)。
EIF3G (eukaryotic translation initiation factor 3 subunit G) is a critical component of the eukaryotic initiation factor 3 (eIF3) complex, which plays a central role in initiating protein synthesis by facilitating ribosome assembly and mRNA recruitment during translation. As part of the multi-subunit eIF3 complex, EIF3G contributes to stabilizing the interaction between the 40S ribosomal subunit and initiator tRNA, as well as regulating the scanning and selection of start codons. The eIF3 complex is also implicated in post-transcriptional gene regulation, stress responses, and disease pathways, including cancer and viral infection.
Antibodies targeting EIF3G are essential tools for studying its expression, localization, and molecular interactions in both physiological and pathological contexts. These antibodies are widely used in techniques such as Western blotting, immunoprecipitation, immunofluorescence, and co-immunoprecipitation to investigate eIF3G’s role in translation initiation, its association with other eIF3 subunits, and its potential dysregulation in diseases. For example, studies have linked altered eIF3G expression to tumor progression, viral replication mechanisms, and neurological disorders, making it a biomarker of interest. Researchers also utilize EIF3G antibodies to explore post-translational modifications, such as phosphorylation, that modulate its function. Validated antibodies with high specificity are critical to ensure accurate detection, given the structural complexity of the eIF3 complex and sequence homology among subunits.
×
