| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/150-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | IPPI2 |
| WB Predicted band size | 27 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human IDI2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于IDI2抗体的模拟参考文献示例(注：部分信息为示例性概括，建议通过学术数据库核实具体文献)：
1. **文献名称**：*IDI2 as a novel cancer/testis antigen: Immunohistochemical analysis in human tumors*
**作者**：Smith A, et al.
**摘要**：研究报道了IDI2在多种癌症组织中的特异性表达，并开发了针对IDI2的多克隆抗体，通过免疫组化验证其在睾丸癌和肺癌中的高表达，提示其作为肿瘤标志物的潜力。
2. **文献名称**：*Functional characterization of IDI2 in isoprenoid biosynthesis using gene knockout and antibody-based detection*
**作者**：Lee J, et al.
**摘要**：利用CRISPR/Cas9敲除IDI2基因，结合特异性抗体进行Western blot和免疫荧光实验，揭示IDI2在胆固醇合成通路中的补偿作用及其与IDI1的功能差异。
3. **文献名称**：*Development of a monoclonal antibody against human IDI2 for targeted therapy screening*
**作者**：Wang Y, et al.
**摘要**：研究团队成功制备了抗人IDI2的单克隆抗体，并验证其高特异性和亲和力，进一步应用于高通量药物筛选，探索IDI2抑制剂在癌症治疗中的应用。
4. **文献名称**：*IDI2 expression correlates with poor prognosis in hepatocellular carcinoma: A tissue microarray study*
**作者**：Chen H, et al.
**摘要**：通过组织芯片技术和IDI2抗体进行大规模样本分析，发现IDI2在肝细胞癌中过表达且与患者生存率负相关，机制可能涉及肿瘤代谢重编程。
**建议**：实际文献需通过PubMed、Google Scholar等平台以“IDI2 antibody”、“Isopentenyl-diphosphate delta isomerase 2”为关键词检索，并关注近年发表的抗体验证研究。
The IDI2 antibody targets isopentenyl-diphosphate delta isomerase 2 (IDI2), an enzyme in the mevalonate pathway responsible for synthesizing isoprenoids, essential precursors for cholesterol, steroid hormones, and other lipid-related molecules. IDI2 catalyzes the isomerization of isopentenyl diphosphate (IPP) to dimethylallyl diphosphate (DMAPP), a critical step in generating farnesyl and geranylgeranyl pyrophosphates for protein prenylation. While IDI2 shares functional redundancy with its homolog IDI1. it exhibits tissue-specific expression patterns, with higher levels observed in the brain, liver, and testes.
Research on IDI2 has gained interest due to its potential role in diseases linked to dysregulated cholesterol metabolism, such as neurodegenerative disorders and cancers. Antibodies against IDI2 are primarily used to study its expression, localization, and regulatory mechanisms in cellular and animal models. They enable detection via Western blotting, immunohistochemistry, and immunofluorescence, aiding investigations into how IDI2 contributes to cellular homeostasis or pathology. Recent studies suggest IDI2 may influence tumor progression by modulating prenylation-dependent signaling pathways. However, its distinct physiological roles compared to IDI1 remain under exploration. IDI2 antibodies thus serve as vital tools for unraveling metabolic adaptations in disease contexts and evaluating therapeutic strategies targeting the mevalonate pathway.
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