首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠肌酸激酶同工酶MB(CK-MB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CK-MB含量。CK-MB即肌酸激酶同工酶，是心肌损伤的重要标志物。其释放量与心肌损伤程度相关。在研究机制上，主要通过检测血液中CK-MB水平变化，辅助诊断急性心肌梗死等心脏疾病，评估病情及预后。试剂盒检测范围为1.25 mU/mL-80 mU/mL，适用于心血管疾病机制研究、心肌缺血再灌注损伤模型评估、药物心脏毒性筛选等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：CK-MB
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：1.25 mU/mL-80 mU/mL
灵敏度：0.312 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 80-99 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 86-105 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 82-100 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 82-101 | |||||
回收率：
| The recovery of mouse CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 94-105 | ||||
| EDTA plasma (n=4) | 95 | 89-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mU/mlOD1OD2AverageCorrected 802.9122.7872.8502.684 402.3862.3742.3802.214 201.6171.6641.6411.475 100.8460.8950.8710.705 50.5530.5230.5380.372 2.50.3180.3360.3270.161 1.250.2460.2520.2490.083 00.1640.1680.166 | ||||||
货期：3-5 working days
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