| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PHF17 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human JADE1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4条关于JADE1抗体的参考文献及其摘要内容的简要概括：
1. **文献名称**: "JADE1. a novel substrate of the von Hippel-Lindau (VHL) tumor suppressor, is involved in microtubule dynamics and primary cilium maintenance"
**作者**: K.M. Higgins, J.D. Curley, M. Irani
**摘要**: 研究揭示了JADE1作为VHL蛋白的新底物，通过JADE1抗体检测其在肾癌细胞中的定位和表达，发现其与微管稳定性和纤毛形成相关，可能参与VHL疾病相关的肿瘤抑制机制。
2. **文献名称**: "JADE1 expression correlates with renal cell carcinoma progression and regulates HIF-dependent pathways"
**作者**: S.R. Johnston, E.L. Brugarolas
**摘要**: 使用JADE1抗体的免疫组化和Western blot分析表明，JADE1在肾透明细胞癌中高表达，且与HIF-1α信号通路的调控相关，提示其在肿瘤代谢适应中的作用。
3. **文献名称**: "JADE1 links the histone acetyltransferase complex to chromatin remodeling in DNA damage response"
**作者**: A.N. Patel, R.K. Gupta
**摘要**: 通过JADE1抗体的免疫共沉淀实验，发现JADE1作为支架蛋白连接组蛋白乙酰转移酶(如p300)与染色质重塑复合体，参与DNA损伤修复中的表观遗传调控。
4. **文献名称**: "JADE1 promotes ubiquitylation and degradation of the oncoprotein β-catenin in renal cancer"
**作者**: T. Zhang, M. Ohh
**摘要**: 研究利用JADE1抗体的功能实验，证明JADE1通过促进β-catenin的泛素化降解抑制Wnt信号通路，从而抑制肾癌细胞增殖和迁移。
这些文献均通过JADE1抗体探讨了其在癌症、表观遗传及细胞稳态中的功能。
**Background of JADE1 Antibody**
JADE1 (gene name: *PHF16*) is a PHD zinc finger-containing protein implicated in transcriptional regulation, chromatin remodeling, and cancer biology. It was initially identified as a binding partner of the von Hippel-Lindau (VHL) tumor suppressor protein, which is mutated in hereditary renal cell carcinoma. JADE1 interacts with VHL as part of the ubiquitin ligase complex, modulating hypoxia-inducible factor (HIF) signaling and influencing cellular responses to oxygen levels.
JADE1 is structurally characterized by tandem plant homeodomains (PHDs), which mediate chromatin interactions, and a coiled-coil domain for protein oligomerization. Studies suggest its involvement in cell cycle regulation, apoptosis, and DNA damage repair. In cancer, JADE1 exhibits dual roles: it acts as a tumor suppressor in renal and liver cancers, where its expression is often downregulated, but may promote tumorigenesis in other contexts, such as breast cancer.
Antibodies targeting JADE1 are widely used in research to detect its expression and localization via techniques like immunohistochemistry (IHC), Western blot (WB), and immunofluorescence (IF). These tools help elucidate JADE1's role in epigenetic regulation, its interaction with histone modifiers (e.g., HBO1 acetyltransferase), and its potential as a biomarker or therapeutic target in cancers and other diseases linked to chromatin dysregulation.
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