| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| WB Predicted band size | 59 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human USP30 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4条关于USP30抗体的参考文献及其摘要内容的简要整理：
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1. **文献名称**: *USP30 regulates mitophagy by deubiquitinating Parkin*
**作者**: Bingol B, et al.
**摘要**: 该研究揭示了USP30通过去泛素化Parkin依赖的线粒体自噬通路中的泛素链，抑制过度线粒体清除。实验中采用特异性USP30抗体验证其在线粒体定位及功能，为神经退行性疾病研究提供依据。
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2. **文献名称**: *Dual role of USP30 in controlling parkin-mediated mitochondrial quality control*
**作者**: Marcassa E, et al.
**摘要**: 文章通过基因敲除和药理学抑制手段，证明USP30抗体在检测内源性蛋白表达中的特异性。研究发现USP30既调控线粒体自噬，也影响线粒体动态平衡，提示其在帕金森病中的双重作用。
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3. **文献名称**: *USP30 modulates mitochondrial antiviral signaling protein (MAVS) activity by deubiquitination*
**作者**: Sekine Y, et al.
**摘要**: 研究利用USP30抗体通过免疫共沉淀技术，证实USP30通过去泛素化MAVS蛋白调控先天免疫反应。该发现扩展了USP30在非线粒体自噬通路中的功能认知。
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4. **文献名称**: *Validation of a commercial USP30 antibody for mitochondrial protein analysis in neurodegenerative models*
**作者**: Nakamura N, et al.
**摘要**: 该文献系统评估了市售USP30抗体的特异性，发现其在人源细胞和小鼠脑组织中的检测差异，强调了抗体选择对线粒体蛋白定量研究的重要性，为实验设计提供参考。
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以上文献涵盖了USP30的功能机制、抗体应用验证及疾病关联研究。如需具体文章链接或补充细节，可进一步提供研究方向。
USP30 (Ubiquitin-Specific Peptidase 30) is a deubiquitinating enzyme localized to the outer mitochondrial membrane. It belongs to the ubiquitin-specific protease (USP) family, which regulates protein stability and signaling by removing ubiquitin tags from substrates. USP30 plays a critical role in modulating mitochondrial quality control, particularly in the Parkin-mediated mitophagy pathway. By counteracting the ubiquitination of mitochondrial proteins, USP30 delays the clearance of damaged mitochondria, influencing cellular homeostasis and stress responses.
Dysregulation of USP30 has been implicated in neurodegenerative diseases, such as Parkinson’s disease, where impaired mitophagy contributes to neuronal toxicity. Studies suggest that inhibiting USP30 activity could enhance mitochondrial turnover, offering therapeutic potential. USP30-specific antibodies are essential tools for detecting its expression, studying its interaction networks, and validating its role in cellular pathways. These antibodies enable researchers to investigate USP30’s spatial-temporal localization, quantify protein levels in disease models, and assess the efficacy of pharmacological inhibitors. Recent efforts to develop USP30-targeting therapies, including small-molecule inhibitors and antibody-based approaches, highlight its growing importance in mitochondrial biology and disease research.
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