| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| WB Predicted band size | 87 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human CUL2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CUL2抗体的3篇参考文献示例(注：文献为模拟示例，实际引用请核实)：
1. **"CUL2-mediated ubiquitination in renal cell carcinoma pathogenesis"**
*作者：Smith J, et al.*
**摘要**：研究揭示了CUL2在肾细胞癌中通过调控VHL蛋白复合物促进肿瘤发生，利用CUL2特异性抗体进行免疫组化及Western blot分析，发现其表达水平与患者预后显著相关。
2. **"Structural insights into the CRL2 ubiquitin ligase complex"**
*作者：Lee H, et al.*
**摘要**：通过冷冻电镜解析CRL2复合物的三维结构，使用CUL2抗体进行免疫共沉淀实验，阐明其底物识别机制及在泛素化通路中的动态组装过程。
3. **"CUL2 antibody validation for hypoxia signaling studies"**
*作者：Zhang Y, et al.*
**摘要**：开发并验证了一种高特异性CUL2单克隆抗体，应用于流式细胞术和免疫荧光，证明CUL2在低氧条件下稳定HIF-1α的作用，为相关靶向治疗提供工具。
建议通过PubMed或Google Scholar以关键词“CUL2 antibody”、“CUL2 ubiquitin ligase”检索最新文献，并筛选实验方法中明确使用该抗体的研究。
The CUL2 antibody is a research tool designed to detect and study Cullin 2 (CUL2), a core component of the Cullin-RING E3 ubiquitin ligase (CRL) complexes. CUL2 functions as a scaffold protein in the CRL2 complex, which typically associates with substrate-recognition adaptors like the von Hippel-Lindau (VHL) tumor suppressor protein. This complex targets specific proteins, such as hypoxia-inducible factor 1α (HIF-1α), for ubiquitination and subsequent proteasomal degradation, playing a critical role in cellular oxygen sensing and tumor suppression. Dysregulation of CUL2 or its associated pathways is implicated in cancers, particularly renal cell carcinoma, where VHL mutations disrupt HIF-1α degradation, promoting angiogenesis and tumor growth.
CUL2 antibodies are widely used in molecular and cancer research to investigate protein expression, localization, and interactions. They enable techniques like Western blotting, immunohistochemistry (IHC), and co-immunoprecipitation (Co-IP) to explore CUL2's role in cellular processes, including cell cycle regulation, DNA repair, and response to hypoxia. Commercially available CUL2 antibodies vary in specificity, targeting different epitopes or post-translational modifications. Researchers must validate these antibodies for specific applications using appropriate controls, such as knockout cell lines, to ensure reliability. Understanding CUL2's function and regulation through antibody-based studies contributes to insights into disease mechanisms and potential therapeutic strategies targeting ubiquitin-proteasome pathways.
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