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产品描述：人封闭蛋白1(CLDN1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CLDN1含量。CLDN1是一种紧密连接蛋白，参与上皮细胞间的粘附和细胞极性维持。其在多种肿瘤中表达异常，与肿瘤的侵袭和转移密切相关。研究显示，CLDN1的表达水平与肿瘤的预后相关，可作为诊断和治疗靶点。其研究机制涉及细胞信号转导、细胞间通讯和肿瘤微环境等。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，可用于探索CLDN1在细胞极性调控、组织屏障完整性研究、肿瘤微环境分析等领域的生物学功能，或在体外模型中评估药物、基因编辑等因素对CLDN1表达的影响，为分子机制研究及潜在治疗策略开发提供数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CLDN1; CLD1; SEMP1; UNQ481/PRO944; Claudin-1; Senescence-associated epithelial membrane protein
缩写：CLDN1
Uniprot No.：O95832
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CLDN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 87-96 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 95-100 | |||||
| 1:4 | Average % | 101 | ||||
| Range % | 94-105 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 90-96 | |||||
回收率：
| The recovery of human CLDN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 86-97 | ||||
| EDTA plasma (n=4) | 99 | 93-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.2472.4122.3302.1895001.6501.7051.6781.5372500.9981.0021.0000.8591250.5860.6010.5940.45362.50.3750.4030.3890.24831.20.2620.2490.2560.11515.60.1880.2010.1950.05400.1400.1420.141 | ||||||
货期：3-5 working days
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