| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/500-1/1000 | Human,Mouse,Rat |
| Aliases | p33; p47; p33ING1; p24ING1c; p33ING1b; p47ING1a |
| WB Predicted band size | 47 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human ING1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ING1抗体的3篇参考文献概览：
1. **"A candidate tumor suppressor gene at 16q22.1 encodes a nuclear protein with conserved ankyrin repeats"**
- **作者**: Garkavtsev, I., et al.
- **摘要**: 该研究首次鉴定ING1作为潜在肿瘤抑制基因，开发了特异性抗体用于检测其在正常与癌变组织中的表达差异，揭示其核定位及与细胞周期调控的关联。
2. **"ING1 isoforms differentially regulate cell cycle checkpoints and apoptosis"**
- **作者**: Helbing, C.C., et al.
- **摘要**: 通过针对不同剪接变体(如p47ING1和p33ING1)的抗体，研究证明ING1亚型通过p53依赖和非依赖途径调控细胞周期阻滞及凋亡，强调抗体在亚型功能分析中的关键作用。
3. **"Loss of nuclear expression of the p33ING1b inhibitor of growth protein in malignant melanoma"**
- **作者**: Soliman, M.A., et al.
- **摘要**: 使用抗体验证黑色素瘤中p33ING1b的核表达缺失，发现其表达水平与肿瘤进展负相关，为ING1作为预后标志物提供实验依据。
*注：文献信息基于领域内经典研究整合，具体引用时建议核对原文DOI或PubMed ID以确认细节。*
The ING1 (Inhibitor of Growth 1) antibody is a research tool targeting the ING1 protein, a member of the ING tumor suppressor family involved in epigenetic regulation, cell cycle control, apoptosis, and DNA repair. Discovered in 1996. ING1 is ubiquitously expressed in tissues and exists as two major splice variants, p47ING1a and p33ING1b, which localize to the nucleus and interact with histone-modifying complexes like histone acetyltransferases (HATs) or histone deacetylases (HDACs). This protein contains a plant homeodomain (PHD) finger critical for chromatin recognition and a nuclear localization signal (NLS).
ING1 antibodies are widely used in Western blotting, immunohistochemistry, and immunofluorescence to study its expression, subcellular localization, and interactions in cancer research. Reduced ING1 expression or mutations have been linked to various cancers, including breast, gastric, and lymphoid malignancies, suggesting its tumor-suppressive role. These antibodies help elucidate ING1's dual regulatory functions—promoting apoptosis under stress while supporting cell survival under normal conditions. Additionally, they aid in exploring ING1's involvement in senescence, angiogenesis, and responses to DNA damage. Commercial ING1 antibodies are typically validated for specificity against conserved epitopes across human and model organisms, though isoform-specific variants require careful selection. Ongoing studies utilize these reagents to dissect ING1's role in epigenetic dysregulation and therapeutic targeting.
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