| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| WB Predicted band size | 181 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human IQGAP2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于IQGAP2抗体的3篇文献概览：
1. **文献名称**: *IQGAP2 suppresses cell migration and phagocytosis in hepatocellular carcinoma*
**作者**: White et al.
**摘要**: 研究通过免疫组化(使用IQGAP2特异性抗体)发现IQGAP2在肝细胞癌中表达下调，其缺失促进肿瘤细胞迁移和侵袭，提示其作为抑癌基因的潜在作用。
2. **文献名称**: *Distinct roles of IQGAP1 and IQGAP2 in modulating Rho GTPase signaling*
**作者**: Briggs & Sacks
**摘要**: 利用IQGAP2抗体进行蛋白质互作分析，揭示IQGAP2通过调控RhoA/ROCK通路影响细胞骨架重组，区别于IQGAP1对Cdc42的调控功能。
3. **文献名称**: *IQGAP2 deficiency promotes intestinal tumorigenesis in APC-mutant mice*
**作者**: Nakamura et al.
**摘要**: 通过Western blot(使用IQGAP2抗体)发现，IQGAP2缺失加速APC突变小鼠的结肠癌进展，表明其与Wnt/β-catenin信号异常相关。
4. **文献名称**: *The role of IQGAP2 in metabolic disorders and cancer*
**作者**: Schmidt & Rojas
**摘要**: 综述总结了IQGAP2在代谢调控和癌症中的双重作用，强调其抗体在临床样本中检测表达水平对预后的评估价值。
这些文献均通过IQGAP2抗体探究其在肿瘤机制中的功能，涵盖肝癌、结肠癌等模型。
The IQGAP2 antibody is a crucial tool for studying the IQ motif-containing GTPase-activating protein 2 (IQGAP2), a member of the IQGAP family of scaffold proteins. IQGAP2. encoded by the *IQGAP2* gene in humans, shares structural homology with IQGAP1 and IQGAP3. including conserved domains such as Ras-GAP-related, calponin homology, and IQ motifs. Unlike IQGAP1. which is ubiquitously expressed, IQGAP2 is predominantly found in tissues like the liver, pancreas, and gastrointestinal tract. It regulates cytoskeletal dynamics, cell adhesion, and signaling pathways by interacting with Rho GTPases (e.g., Rac1. Cdc42) and other partners, though it lacks intrinsic GTPase-activating activity for Ras.
Research highlights IQGAP2's role as a potential tumor suppressor. Reduced IQGAP2 expression correlates with hepatocellular carcinoma, colorectal cancer, and other malignancies, possibly through dysregulation of β-catenin signaling. Its loss may promote tumor progression, contrasting with IQGAP1's oncogenic functions. IQGAP2 antibodies enable detection of protein expression, localization, and post-translational modifications via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Specificity is critical, as cross-reactivity with IQGAP1 or IQGAP3 must be minimized. These antibodies aid in elucidating IQGAP2's physiological roles in tissue homeostasis and its pathological contributions to cancer and metabolic disorders, offering insights into therapeutic targeting. Validation using knockout models or siRNA is recommended to confirm antibody reliability in experimental settings.
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