首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠Ⅱ型胶原(Col Ⅱ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Col Ⅱ含量。小鼠Ⅱ型胶原（Col Ⅱ）是软骨的主要成分，具有维持软骨组织完整性和促进软骨细胞生长的功能。其研究背景涉及基因工程技术，通过毕赤酵母表达重组Col Ⅱ，用于凝血和组织再生。研究机制包括Col Ⅱ在促进凝血、成纤维细胞分化中的应用，以及其在治疗骨关节炎等疾病中的作用。试剂盒检测范围为1.87 ng/mL-120 ng/mL，适用于科研领域中小鼠关节炎模型评估、软骨发育机制研究或退行性关节疾病中Col Ⅱ代谢动态分析，为体外实验提供高特异性的定量检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Collagen Type 2,Col 2,_x000D_ Collagen Type II,Col II
缩写：Col Ⅱ
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：1.87 ng/mL-120 ng/mL
灵敏度：0.46 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 81-92 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 94-105 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-100 | |||||
回收率：
| The recovery of mouse Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 82-93 | ||||
| EDTA plasma (n=4) | 104 | 95-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 1202.6682.5112.5902.401 602.1492.1002.1251.936 301.4591.4451.4521.263 150.8580.8320.8450.656 7.50.5400.5550.5480.359 3.750.3830.3890.3860.197 1.870.2920.2980.2950.106 00.1880.1900.189 | ||||||
货期：3-5 working days
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