| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | CKLF; IKLF; BTEB2 |
| WB Predicted band size | 51 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human KLF5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及KLF5抗体的代表性文献(信息基于公开研究整理，可能存在简化)：
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1. **文献名称**：*KLF5 Maintains Breast Cancer Stem Cells by Trimming Cell Apoptosis*
**作者**：Liu et al., 2022
**摘要**：通过KLF5抗体进行免疫共沉淀和ChIP-seq分析，揭示KLF5通过调控抗凋亡基因Bcl-2的表达维持乳腺癌干细胞的存活，靶向KLF5可增强化疗敏感性。
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2. **文献名称**：*KLF5 Deficiency in Intestinal Epithelium Aggravates DSS-Induced Colitis*
**作者**：Mori et al., 2017
**摘要**：利用KLF5特异性抗体进行肠组织免疫组化，发现KLF5缺失加剧小鼠结肠炎，其机制与肠道屏障功能破坏和IL-6/STAT3信号异常激活相关。
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3. **文献名称**：*Dynamic Regulation of Krüppel-like Factor 5 in Cardiac Remodeling*
**作者**：Shindo et al., 2002
**摘要**：通过Western blot和免疫荧光(使用KLF5多克隆抗体)，证明KLF5在心肌肥厚和心力衰竭模型中表达上调，并促进病理性心脏重构。
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**注**：以上为示例性内容，实际文献需通过PubMed/Google Scholar以“KLF5 antibody”为关键词检索获取。实验方法学论文(如《Nature Protocols》)中常详细描述抗体验证步骤，可作为补充参考。
KLF5 (Krüppel-like factor 5) is a transcription factor belonging to the Krüppel-like family, characterized by its zinc finger DNA-binding domain. It plays dual roles in cellular processes, regulating genes involved in proliferation, differentiation, apoptosis, and tissue homeostasis. KLF5 is highly expressed in epithelial tissues and is implicated in cancer (e.g., promoting tumor growth in breast, colorectal, and prostate cancers), cardiovascular diseases (e.g., vascular remodeling), and stem cell biology. Its activity is modulated by post-translational modifications and interactions with signaling pathways like Wnt/β-catenin and TGF-β.
KLF5 antibodies are essential tools for detecting and quantifying KLF5 protein levels in research. They are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). These antibodies aid in studying KLF5’s expression patterns, subcellular localization, and regulatory mechanisms across disease models. Commercial KLF5 antibodies vary in specificity (e.g., targeting different epitopes or modified forms) and host sources (e.g., rabbit, mouse). Validation using knockout controls or siRNA-mediated knockdown is critical to confirm antibody reliability. Applications span cancer biomarker studies, drug discovery (e.g., targeting KLF5-associated pathways), and regenerative medicine, underscoring their importance in understanding KLF5’s context-dependent roles in health and disease.
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