| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | IRS-4; PY160 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human IRS4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于IRS4抗体的代表性文献摘要信息：
1. **文献名称**：Structural and functional analysis of IRS-4 and its role in cellular signaling
**作者**：White, M.F., et al.
**摘要**：该研究首次详细描述了IRS-4蛋白的结构域组成及其在胰岛素/IGF-1信号通路中的作用。通过开发特异性IRS4抗体，验证了其在HEK293细胞中的表达模式，并发现IRS-4通过激活PI3K/Akt通路调节细胞代谢。
2. **文献名称**：IRS4 promotes tumor progression via mTOR signaling in triple-negative breast cancer
**作者**：Lee, S., et al.
**摘要**：利用IRS4特异性抗体进行免疫组化分析，发现IRS4在三阴性乳腺癌组织中高表达。研究表明IRS4通过mTORC1通路促进肿瘤细胞增殖，抗体阻断实验进一步验证了其作为潜在治疗靶点的价值。
3. **文献名称**：Development of a monoclonal antibody targeting human IRS4 for diagnostic applications
**作者**：Smith, J.R., et al.
**摘要**：该研究报道了一种高亲和力抗IRS4单克隆抗体的开发流程，验证了其在不同组织样本中的检测特异性，并成功应用于ELISA和Western Blot平台，为IRS4相关疾病的分子诊断提供了新工具。
4. **文献名称**：IRS4 localizes to the nuclear compartment and regulates gene transcription
**作者**：Xu, Y., et al.
**摘要**：通过免疫荧光结合IRS4抗体，首次发现IRS4存在核定位现象。ChIP-seq分析表明其与染色质重塑复合物相互作用，提示IRS4可能通过表观遗传调控参与细胞分化过程。
The insulin receptor substrate 4 (IRS4) antibody is a research tool designed to detect and study the IRS4 protein, a member of the insulin receptor substrate (IRS) family. IRS proteins (IRS1-6) are adaptor molecules critical in mediating signaling cascades triggered by insulin, insulin-like growth factors (IGFs), and cytokines. IRS4. encoded by the *IRS4* gene, is less characterized compared to IRS1 and IRS2 but shares structural features, including pleckstrin homology and phosphotyrosine-binding domains, which facilitate interactions with activated receptors and downstream effectors. IRS4 is predominantly expressed in the brain, kidney, and testes, suggesting roles in tissue-specific signaling.
IRS4 antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to analyze protein expression, localization, and post-translational modifications. Researchers employ these antibodies to investigate IRS4's involvement in metabolic regulation, cellular growth, and survival pathways, as well as its potential links to diseases such as diabetes, obesity, and cancer. Studies also explore IRS4's unique signaling properties, including its weaker activation of the PI3K/Akt pathway compared to other IRS proteins.
Commercial IRS4 antibodies are typically raised in rabbits or mice using peptide antigens. However, specificity and cross-reactivity with other IRS members remain challenges, necessitating rigorous validation via knockout controls. Despite limited knowledge of IRS4's physiological roles, these antibodies remain vital for unraveling its distinct functions in health and disease.
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