| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | IRS-2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human IRS2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与IRS2抗体相关的参考文献，简要概括其内容：
1. **文献名称**：*"Role of IRS-2 in β-cell development, insulin secretion and insulin receptor signalling"*
**作者**：Morris DL, et al. (2006)
**摘要**：该研究利用IRS-2敲除小鼠模型，通过Western blot和免疫组化分析，发现IRS-2对β细胞增殖和胰岛素分泌至关重要，并揭示了其在肝脏和胰腺中的信号通路差异。
2. **文献名称**：*"Adipose tissue-specific IRS-2 knockout mice exhibit metabolic phenotypes distinct from whole-body IRS-2 deficiency"*
**作者**：Kubota N, et al. (2004)
**摘要**：通过组织特异性敲除技术结合IRS-2抗体检测，研究发现脂肪组织中IRS-2缺失导致全身胰岛素抵抗，但不同于全身性敲除，提示组织间IRS-2功能的特异性。
3. **文献名称**：*"Disruption of IRS-2 causes type 2 diabetes in mice"*
**作者**：Withers DJ, et al. (2000)
**摘要**：利用IRS-2抗体进行蛋白表达分析，证实IRS-2缺陷小鼠出现胰岛素抵抗和β细胞功能衰竭，首次明确IRS-2在2型糖尿病发病中的核心作用。
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**备注**：以上文献为示例，实际引用需核对PubMed或期刊数据库获取完整信息。IRS-2抗体相关研究多聚焦于代谢疾病与癌症机制，建议结合具体研究方向筛选文献。
The insulin receptor substrate 2 (IRS2) antibody is a crucial tool for studying the IRS2 protein, a central mediator in insulin and insulin-like growth factor (IGF) signaling pathways. IRS2 belongs to the IRS family of cytoplasmic adaptor proteins, which transmit signals from activated cell surface receptors to intracellular effectors. It plays a vital role in regulating metabolic homeostasis, cell growth, survival, and differentiation. Dysregulation of IRS2 is implicated in metabolic disorders (e.g., type 2 diabetes, obesity), cancer progression, and neurodegenerative conditions like Alzheimer's disease.
IRS2 antibodies are designed to detect specific epitopes of the IRS2 protein, enabling researchers to study its expression, localization, and post-translational modifications. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry, immunofluorescence, and co-immunoprecipitation. Polyclonal and monoclonal variants are available, often raised in hosts like rabbits or mice. Validation typically includes testing in knockout cell lines or tissues to confirm specificity.
Research applications focus on IRS2's dual role in metabolism and oncogenesis. For example, IRS2 overexpression is linked to cancer cell proliferation and therapy resistance, while its deficiency correlates with insulin resistance. When using IRS2 antibodies, experimental conditions (e.g., tissue type, fixation methods) must be optimized to avoid cross-reactivity with homologous proteins like IRS1. Commercial antibodies often provide datasheets detailing validated species reactivity and recommended protocols.
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