首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子Ⅰ型胶原(Col Ⅰ)酶联免疫试剂盒双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Col Ⅰ含量。试剂盒检测范围为0.156 ng/mL-10 ng/mL，本试剂盒通过高特异性抗体对实现靶标捕获与信号放大，适用于体外研究胶原代谢调控机制、评估纤维化疾病模型进展或探索组织工程材料中胶原沉积规律等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Collagen Type 1,Col 1,_x000D_ Collagen Type I,Col I
缩写：Col Ⅰ
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Col Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:8 | Average % | 104 | ||||
| Range % | 100-109 | |||||
回收率：
| The recovery of rabbit Col Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 90-96 | ||||
| EDTA plasma (n=4) | 99 | 94-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 102.0132.1362.0751.935 51.7491.7051.7271.587 2.51.3881.4981.4431.303 1.251.0651.1541.1100.970 0.6250.7540.7880.7710.631 0.3120.5130.5320.5230.383 0.1560.3120.3310.3220.182 00.1390.1410.140 | ||||||
货期：3-5 working days
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