首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子Ⅳ型胶原(Col Ⅳ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Col 含量。试剂盒检测范围为12.5 ng/mL-800 ng/mL，可应用于基础研究领域，如探究肾脏、肝脏等器官纤维化模型中的基底膜代谢异常，分析肿瘤微环境中基质重构机制，或评估血管病变相关疾病模型中Col IV的动态变化。科研人员可通过血清/血浆样本实现纵向监测，或利用组织匀浆进行局部表达水平解析，为组织工程、病理机制及药物干预研究提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Collagen Type 4(COL 4),_x000D_ Collagen Type IV(COL IV)
缩写：Col
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：12.5 ng/mL-800 ng/mL
灵敏度：3.12 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit COL Ⅳ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 84-91 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 95-104 | |||||
| 1:8 | Average % | 86 | ||||
| Range % | 80-94 | |||||
回收率：
| The recovery of rabbit COL Ⅳ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 81-92 | ||||
| EDTA plasma (n=4) | 93 | 87-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 8002.7342.5342.6342.541 4002.0552.1352.0952.002 2001.3481.3241.3361.243 1000.6770.6690.6730.580 500.4120.4210.4170.324 250.2780.2740.2760.183 12.50.1510.1540.1530.060 00.0930.0920.093 | ||||||
货期：3-5 working days
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