首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠和肽素(copeptin)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的copeptin含量。copeptin是精氨酸加压素原的C末端片段，稳定性好。其背景在于检测它可间接反映精氨酸加压素水平。研究机制方面，在一些疾病如心血管病、内分泌疾病等中，copeptin水平会发生变化，可作为疾病诊断、病情评估和预后判断的生物标志物。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，该产品适用于实验室研究中对大鼠模型在病理生理状态下的和肽素水平动态监测，例如心血管疾病机制探索、应激反应评估或肾脏功能相关研究，为深入解析相关疾病的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：copeptin
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat copeptin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 89-102 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 96-106 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 90-102 | |||||
回收率：
| The recovery of rat copeptin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 104 | 98-108 | ||||
| EDTA plasma (n=4) | 88 | 82-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.9162.8152.8662.74220002.1212.2322.1772.05310001.3901.4011.3961.2725000.8000.8100.8050.6812500.4580.4470.4530.3291250.2630.2520.2580.13462.50.1730.1710.1720.04800.1240.1230.124 | ||||||
货期：3-5 working days
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