Mouse Corticosterone,CORT ELISA Kit

大包装询价

货号: IPD-E07969m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠皮质酮/肾上腺酮(CORT)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CORT含量。试剂盒检测范围为15.6 ng/ml-1000 ng/ml，适用于体外分析多种生物样本中的CORT水平，尤其适用于评估应激反应模型、慢性代谢疾病研究或药物干预实验中的肾上腺功能状态，可满足实验室对小鼠模型应激水平、激素代谢动态及病理生理机制研究的精准检测需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
N/A
缩写：
CORT
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
15.6 ng/ml-1000 ng/ml.
灵敏度：
3.9 ng/ml.
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Signal Transduction
测定原理：
quantitative
测定方法：
Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CORT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-92
1:2	Average %	87
	Range %	81-95
1:4	Average %	85
	Range %	82-93
1:8	Average %	88
	Range %	82-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average
1000	0.150	0.144	0.147
500	0.185	0.182	0.183
250	0.261	0.248	0.254
125	0.389	0.400	0.395
62.5	0.642	0.621	0.632
31.25	1.004	1.053	1.028
15.6	1.410	1.384	1.397
0	2.166	2.125	2.145

A micro ELISA plate --- The 96-well plate has been pre-coated with goat anti-rabbit CORT antibody.
Six vials lyophilized standard (0.5 ml/bottle) --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
Anti-mouse CORT Antibody (1 x 6 ml) --- Act as the detection antibody.
HRP-conjugate (1 x 6 ml) --- HRP catalyzes TMB substrate to elicit a chromogenic reaction.
Wash Buffer (20 x concentrate) (1 x 15 ml) --- Wash away unbound or free substances.
Substrate A (1 x 7 ml) --- Mix with substrate B, and the TMB is catalyzed by HRP to elicit a chromogenic signal.

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm to 630 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.