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产品描述：鸡皮质酮/肾上腺酮(CORT)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的CORT含量。试剂盒检测范围为0.5 ng/mL-20 ng/mL，广泛应用于禽类应激生物学研究、养殖环境优化评估、神经内分泌调控模型构建等领域，为实验室开展家禽生理状态监测、行为生态学分析及激素代谢通路探索提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：CORT
种属：Gallus gallus (Chicken)
样本类型：serum, plasma
检测范围：0.5 ng/mL-20 ng/mL
灵敏度：0.5 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken CORT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 94-103 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-101 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 87-98 | |||||
回收率：
| The recovery of chicken CORT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 84 | 80-88 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 200.1610.1490.155 100.2450.2590.252 50.4080.3940.401 20.6580.6090.634 10.9590.9040.932 0.51.3411.2761.309 01.8421.7721.807 | ||||||
货期：3-5 working days
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