首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠羧肽酶B(CPB1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CPB1含量。CPB1，即羧肽酶B1，是一种胰腺特异性蛋白，参与蛋白质消化。研究显示，CPB1在胰腺癌全切术后表达上调，可能与疾病复发和转移相关。研究机制涉及基因表达调控、蛋白质功能分析等。试剂盒检测范围为12.5 ng/mL-800 ng/mL，适用于科研场景下对大鼠CPB1的表达水平分析，例如研究胰腺外分泌功能、营养代谢调控机制或建立相关疾病模型时的生物标志物检测，为探索CPB1在生理病理过程中的作用提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cpb1 ELISA kit; CpbCarboxypeptidase B ELISA kit; EC 3.4.17.2 ELISA kit
缩写：CPB1
Uniprot No.：P19223
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：12.5 ng/mL-800 ng/mL
灵敏度：3.12 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CPB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 95-110 | |||||
| 1:2 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 92-103 | |||||
| 1:8 | Average % | 105 | ||||
| Range % | 100-112 | |||||
回收率：
| The recovery of rat CPB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 84-93 | ||||
| EDTA plasma (n=4) | 93 | 88-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected8002.5962.7142.6552.5364002.2122.1422.1772.0582001.4551.5241.4901.3711000.7890.7650.7770.658500.4210.4480.4350.316250.2430.2530.2480.12912.50.1650.1750.1700.05100.1200.1180.119 | ||||||
货期：3-5 working days
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