首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠铜蓝蛋白(CP/CER)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CP含量。小鼠铜蓝蛋白（CP/CER）是一种多铜氧化酶和抗氧化剂，主要在肝脏中合成，参与铜和铁的代谢平衡，具有抗氧化活性。研究发现，铜蓝蛋白在阿尔茨海默症、帕金森病等神经退行性疾病及糖尿病、肥胖等代谢性疾病中发挥重要作用，其功能可能与促进神经细胞生长、改善学习记忆能力、调节脂质代谢和抗氧化应激有关。试剂盒检测范围为31.2 ng/mL-2000 ng/mL，适用于体外研究，可用于铜代谢机制探索、肝脏功能评估、炎症性疾病模型建立等科研领域，支持小鼠血清（1:10稀释）和细胞上清（直接检测）等多种样本类型。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cp ELISA Kit; Ceruloplasmin ELISA Kit; EC 1.16.3.1 ELISA Kit; Ferroxidase ELISA Kit
缩写：CP
Uniprot No.：Q61147
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：31.2 ng/mL-2000 ng/mL
灵敏度：7.8 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CP/CER in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 95 | ||||
| Range % | 83-106 | |||||
| 1:2000 | Average % | 92 | ||||
| Range % | 83-104 | |||||
| 1:4000 | Average % | 95 | ||||
| Range % | 91-102 | |||||
| 1:8000 | Average % | 93 | ||||
| Range % | 87-99 | |||||
回收率：
| The recovery of mouse CP/CER spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-99 | ||||
| EDTA plasma (n=4) | 92 | 85-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 20000.1030.1050.104 10000.1690.1710.170 5000.3010.3090.305 2500.5750.5820.579 1251.2941.3061.300 62.51.8551.8641.860 31.22.5362.5412.539 02.8792.8872.883 | ||||||
货期：3-5 working days
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