首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠皮质酮/肾上腺酮(CORT)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CORT含量。CORT即皮质酮，是一种重要的糖皮质激素。它参与机体应激反应与代谢调节等生理过程。研究机制上，主要通过与细胞内糖皮质激素受体结合，调控相关基因表达，影响细胞功能，在神经、内分泌等多系统中发挥关键作用。试剂盒检测范围为0.2 ng/mL-40 ng/mL，支持科研人员开展神经内分泌机制研究、慢性应激模型评估、代谢综合征相关实验及药物干预效果验证等领域的探索，为行为学实验、神经药理学研究和激素调控机制探索提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：CORT
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.2 ng/mL-40 ng/mL
灵敏度：0.1 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CORT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-91 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:8 | Average % | 105 | ||||
| Range % | 100-112 | |||||
回收率：
| The recovery of rat CORT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 92 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 400.1380.1320.135 100.2390.2580.249 3.20.5040.5090.507 0.80.9391.0090.974 0.21.4191.4241.422 02.1542.1592.157 | ||||||
货期：3-5 working days
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