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产品描述：猪皮质酮/肾上腺酮(CORT)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的CORT含量。CORT（皮质酮）属糖皮质激素。在生理背景中，它参与机体应激反应等调节。研究机制方面，主要聚焦其与受体结合影响基因表达，在神经、内分泌等多个系统中发挥作用，还涉及对代谢、免疫功能的调控研究。试剂盒检测范围为0.5 ng/mL-20 ng/mL，该产品可应用于科研领域，如评估养殖环境应激对猪生理状态的影响、探究疾病模型中HPA轴功能变化，或用于药理学研究中糖皮质激素代谢调控机制的探索，为动物生理学、兽医学及比较医学研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：CORT
种属：Sus scrofa (Pig)
样本类型：serum, plasma
检测范围：0.5 ng/mL-20 ng/mL
灵敏度：0.5 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig CORT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 84 | |||||
| Range % | 80-88 | ||||||
| 1:2 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
| 1:4 | Average % | 96 | |||||
| Range % | 98-100 | ||||||
| 1:8 | Average % | 90 | |||||
| Range % | 85-94 | ||||||
回收率：
| The recovery of pig CORT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 84-93 | |||||
| EDTA plasma (n=4) | 100 | 95-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2Average 200.3660.3740.370 100.5340.5190.527 50.7810.8210.801 21.2741.3431.309 11.8071.8501.829 0.52.2432.2922.268 02.7812.6352.708 | |||||||
货期：3-5 working days
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