Rabbit Cortisol ELISA Kit

货号: IPD-E06956Rb

Price： ￥3000

48T 96T

数量：

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产品描述：兔子皮质醇(Cortisol)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Cortisol含量。皮质醇，又称压力荷尔蒙，是调节身体对压力反应的关键激素。其分泌受垂体和肾上腺控制，参与调节血压、免疫和新陈代谢。研究皮质醇作为靶点，旨在了解其与压力、疾病及生理过程的关系，以开发新的治疗策略。试剂盒检测范围为62.5 pmol/mL-1000 pmol/mL，该产品适用于科研领域，如动物应激模型研究、药物对肾上腺功能影响的评估，或生理病理状态下皮质醇动态变化的分析，为神经内分泌学、药理学及基础医学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：Cortisol
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pmol/mL-1000 pmol/mL
灵敏度：62.5 pmol/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	81-97
1:2	Average %	89
	Range %	82-98
1:4	Average %	90
	Range %	85-100
1:8	Average %	95
	Range %	89-105

回收率：

The recovery of rabbit Cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-99
EDTA plasma (n=4)	95	89-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pmol/mlOD1OD2Average　10000.1620.1670.165　5000.2680.2750.272　2500.4910.5020.497　1251.0281.0131.021　62.52.0272.0492.038

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