| 纯度 | >80%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRH1 |
| Uniprot No | P02810 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 17-122aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSQDLNEDVSQEDVPLVISDGGDSEQFLD EERQGPPLGGQQSQPSAGDGNQDDGPQQGPPQQGGQQQQGPPPPQGKPQG PPQQGGHPPPPQGRPQGPPQQGGHPRPPR |
| 预测分子量 | 13 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PRH1重组蛋白的模拟参考文献示例(仅供参考，非真实文献)：
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1. **标题**: *"Recombinant PRH1 Expression in E. coli and Its Role in Dental Enamel Protection"*
**作者**: Smith A, et al.
**摘要**: 本研究利用大肠杆菌系统成功表达并纯化了重组PRH1蛋白，证实其在体外能够抑制酸性环境下的牙釉质脱矿，为口腔保健产品的开发提供了潜在分子基础。
2. **标题**: *"Functional Characterization of PRH1 Recombinant Protein in Salivary Antimicrobial Activity"*
**作者**: Lee J, et al.
**摘要**: 通过酵母表达系统获得重组PRH1.发现其能特异性结合口腔致病菌(如变形链球菌)，并抑制生物膜形成，揭示了其在天然免疫防御中的功能。
3. **标题**: *"Optimization of PRH1 Production in Mammalian Cells for Structural Studies"*
**作者**: García-Ramírez M, et al.
**摘要**: 通过哺乳动物细胞表达系统优化PRH1重组蛋白的分泌表达，获得高纯度蛋白用于X射线晶体学研究，解析了其富脯氨酸结构域的独特构象。
4. **标题**: *"PRH1 Recombinant Protein as a Biomarker for Early Oral Cancer Detection"*
**作者**: Chen L, et al.
**摘要**: 研究重组PRH1在口腔癌患者唾液中的表达水平变化，发现其与肿瘤进展相关，提示其作为非侵入性诊断标志物的潜力。
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**注意**：以上文献为模拟示例，实际研究中请通过PubMed、Google Scholar等平台检索真实文献。建议使用关键词“PRH1 recombinant protein”、“PRH1 expression and function”等进行精准查询。
PRH1 recombinant protein is derived from the PRH1 gene, which encodes a proline-rich protein primarily associated with oral biology and salivary function. The PRH1 protein, also known as salivary proline-rich protein 1 or acidic proline-rich protein, is a key component of human saliva, contributing to oral lubrication, enamel pellicle formation, and microbial homeostasis. It is characterized by its high proline content (~25-30%) and post-translational modifications like phosphorylation, which enhance its interaction with hydroxyapatite surfaces in teeth. These properties enable PRH1 to play a dual role: facilitating tooth mineralization and modulating bacterial adhesion, thus influencing oral health and disease susceptibility.
Recombinant PRH1 is produced using heterologous expression systems (e.g., E. coli, mammalian cells) to study its structural and functional properties without relying on native salivary sources. Researchers employ techniques like codon optimization and affinity tag fusion to improve yield and purification efficiency. The recombinant protein retains critical domains for calcium-binding and bacterial agglutination, making it valuable for investigating mechanisms of dental caries, periodontal disease, and oral microbiome dynamics. Recent studies also explore its potential in biomimetic materials for enamel repair and antimicrobial coatings. Limitations in native protein availability and ethical concerns about human saliva collection have further driven the demand for recombinant PRH1 as a standardized research tool. Its applications extend to diagnostic assays, therapeutic development, and as a model for understanding protein-mineral interactions in biomineralization processes.
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